Love J, Gribbin C, Mather C, Sang H
AFRC Roslin Institute Edinburgh, Roslin, Midlothian, U.K.
Biotechnology (N Y). 1994 Jan;12(1):60-3. doi: 10.1038/nbt0194-60.
We have developed a method for production of transgenic chickens by DNA microinjection of chick zygotes followed by ex vivo embryo culture. The fate of plasmid DNA microinjected into the germinal disc of zygotes was analyzed in embryos which survived for at least 12 days in culture. Approximately half of the embryos contained plasmid DNA, 6% at a level equivalent to one copy per cell in all tissues analyzed. Seven chicks, 5.5% of the total number of injected ova, survived to sexual maturity. One of these, a cockerel, transmitted the exogenous DNA to 3.4% of his offspring. These G1 birds have reached sexual maturity and have been bred to produce transgenic offspring, demonstrating that stable transmission of foreign DNA can be obtained by our method.
我们已经开发出一种通过对鸡受精卵进行DNA显微注射并随后进行体外胚胎培养来生产转基因鸡的方法。对显微注射到受精卵胚盘的质粒DNA在培养中存活至少12天的胚胎中的命运进行了分析。大约一半的胚胎含有质粒DNA，在所有分析的组织中，6%的水平相当于每个细胞一个拷贝。7只小鸡（占注射卵总数的5.5%）存活至性成熟。其中一只公鸡将外源DNA传递给了其3.4%的后代。这些G1代鸡已达到性成熟并已进行繁殖以产生转基因后代，这表明通过我们的方法可以实现外源DNA的稳定传递。
