Marine and Highland Bioscience Center, Saga University, 152-1 Shonan-cho, Karatsu, Saga, 847-0021, Japan.
Cytotechnology. 2000 Jul;33(1-3):221-7. doi: 10.1023/A:1008179302906.
We attempted to screen for telomerase inhibitingactivity in vitro from a total of 304 marine algae samples which were collected from various Japancoasts by nonradioisotope telomeric repeatamplification protocol (Non-RI TRAP) assay using humanleukemia MOLT-4 cells. Ten of the MeOH extracts andtwo of the PBS extracts from those algal samplesshowed telomerase inhibiting activity. In particular,the MeOH extract from a green alga, Caulerpasertularioides strongly inhibited telomerase activitywhen added to MOLT-4 cell culture at a level of 1.25%(v/v), suggesting that it may be possible to developa novel anti-cancer agent in view of its specificanti-telomeric property.
我们试图通过非放射性端粒重复扩增协议（Non-RI TRAP）检测，从日本各地采集的 304 种海洋藻类样本中筛选出体外端粒酶抑制活性。用人类白血病 MOLT-4 细胞进行检测，从这些藻类样本的甲醇提取物中，有 10 种和 PBS 提取物中的 2 种表现出端粒酶抑制活性。特别是，甲醇提取物从一种绿藻，Caulerpa sertularioides，当添加到 MOLT-4 细胞培养物中时，在 1.25%（v/v）的水平上强烈抑制端粒酶活性，这表明鉴于其特定的抗端粒性质，可能有可能开发出一种新型抗癌药物。
