甘露糖赤藓糖醇脂质可增加 PC12 嗜铬细胞瘤细胞中的半乳糖神经酰胺含量和神经突生长。
Mannosylerythritol lipid increases levels of galactoceramide in and neurite outgrowth from PC12 pheochromocytoma cells.
机构信息
Tsukuba Life Science Center, RIKEN (The Institute of Physical and Chemical Research), 3-1-1 Koyadai, Tsukuba, Ibaraki, 305-0074, Japan.
出版信息
Cytotechnology. 2000 Jul;33(1-3):247-51. doi: 10.1023/A:1008155111024.
We report here that a microbial extracellular glycolipid,mannosylerythritol lipid (MEL), induces the outgrowth ofneurites from and enhances the activity of acetylcholinesterase(AChE) in PC12 pheochromocytoma cells. Furthermore, treatment ofPC12 cells with MEL increased levels of galactosylceramide(Galbeta1-1'Cer; GalCer). Exposure of PC12 cells to exogenous GalCer caused the dose-dependent outgrowth ofneurites. By contrast, treatment of PC12 cells with nerve growthfactor (NGF) did not increase the level of GalCer in the cells. The neurite-related morphological changes induced by GalCerdifferend from those induced by NGF, indicating differencesbetween the signal transduction pathways triggered by NGF and by GalCer.
我们在这里报告，一种微生物细胞外糖脂，甘露糖赤藓糖醇脂质（MEL），诱导 PC12 嗜铬细胞瘤细胞的轴突生长并增强乙酰胆碱酯酶（AChE）的活性。此外，MEL 处理 PC12 细胞增加半乳糖基神经酰胺（Galβ1-1'Cer；GalCer）的水平。PC12 细胞暴露于外源性 GalCer 引起轴突的剂量依赖性生长。相比之下，用神经生长因子（NGF）处理 PC12 细胞不会增加细胞中 GalCer 的水平。GalCer 诱导的与神经突相关的形态变化与 NGF 诱导的不同，表明 NGF 和 GalCer 触发的信号转导途径之间存在差异。
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