从锯缘青蟹中建立原代细胞培养：锯缘青蟹的原代细胞培养。
Development of primary cell culture from Scylla serrata : Primary cell cultures from Scylla serrata.
机构信息
Department of zoology, Goa University, Taleigao plateau, Goa, 403206, India.
出版信息
Cytotechnology. 2008 Mar;56(3):161-9. doi: 10.1007/s10616-008-9152-1. Epub 2008 Jun 7.
Abstract
This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 x L-15 + crab saline, 2 x L-15 + crab saline, 3 x L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A modified L-15 (3x) medium supported the best survival of hepatopancreatic E, F B, and R cells in in-vitro culture. However granular cells could be maintained for 184 days with L-15 (1x) + crab saline. Fetal bovine serum was not effective additive and hampered cell viability in present study.
摘要
本文首次报道了使用蟹盐水、L-15（Leibovitz）、1 x L-15 + 蟹盐水、2 x L-15 + 蟹盐水、3 x L-15 和柠檬酸盐缓冲液，在无任何血清的情况下从食用蟹锯缘青蟹（Scylla serrata）的肝胰腺中进行原代细胞培养。我们可以分离并维持 E（胚胎细胞）、F（纤维细胞）、B（囊泡细胞）、R（休止细胞）和 G（颗粒细胞）。在接种肝胰腺 E、F、B 和 R 细胞后，它们的存活模式随时间呈现出与颗粒细胞不同的特点。改良的 L-15（3x）培养基在体外培养中最有利于肝胰腺 E、F、B 和 R 细胞的存活。然而，颗粒细胞可以在 L-15（1x）+蟹盐水的条件下维持 184 天。在本研究中，胎牛血清不是有效的添加剂，反而会损害细胞活力。
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