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Fine structural analysis of the epithelial cells in the hepatopancreas of Palaemonetes argentinus (Crustacea, Decapoda, Caridea) in intermoult.阿根廷沼虾（甲壳纲，十足目，虾蛄科）蜕皮间期肝胰腺上皮细胞的超微结构分析
Biocell. 2005 Apr;29(1):25-31.
2
Changes in cell type composition and enzymatic activities in the hepatopancreas of Marsupenaeus japonicus during the moulting cycle.日本囊对虾蜕皮周期中肝胰腺细胞类型组成及酶活性的变化
J Comp Physiol B. 2003 Jun;173(4):355-63. doi: 10.1007/s00360-003-0348-6. Epub 2003 May 8.
3
Development and characterization of primary cell cultures from the hematopoietic tissues of the Dublin Bay prawn, Nephrops norvegicus.挪威龙虾（Nephrops norvegicus）造血组织原代细胞培养物的建立与特性研究
Methods Cell Sci. 2000;22(4):265-75. doi: 10.1023/a:1017971618398.
4
Genetic Diversity and Species-Diagnostic Markers of Mud Crabs (Genus Scylla) in Eastern Thailand Determined by RAPD Analysis.泰国东部锯缘青蟹属（Scylla）锯缘青蟹的遗传多样性及物种诊断标记的随机扩增多态性DNA分析
Mar Biotechnol (NY). 2000 Mar;2(2):180-187. doi: 10.1007/s101269900023.
5
Early attempts at production of prawn cell lines.对虾细胞系生产的早期尝试。
Methods Cell Sci. 1999;21(4):207-12. doi: 10.1023/a:1009806606562.
6
Establishment of cell lines derived from oyster, Crassostrea gigas Thunberg and hard clam, Meretrix lusoria Röding.源自太平洋牡蛎（Crassostrea gigas Thunberg）和文蛤（Meretrix lusoria Röding）的细胞系的建立。
Methods Cell Sci. 1999;21(4):183-92. doi: 10.1023/a:1009829807954.
7
Primary culture of lymphoid, nerve, and ovary cells from Penaeus stylirostris and Penaeus vannamei.来自南美白对虾和凡纳滨对虾的淋巴、神经及卵巢细胞的原代培养。
In Vitro Cell Dev Biol Anim. 1993 Aug;29A(8):620-2. doi: 10.1007/BF02634546.

从锯缘青蟹中建立原代细胞培养：锯缘青蟹的原代细胞培养。

Development of primary cell culture from Scylla serrata : Primary cell cultures from Scylla serrata.

机构信息

Department of zoology, Goa University, Taleigao plateau, Goa, 403206, India.

出版信息

Cytotechnology. 2008 Mar;56(3):161-9. doi: 10.1007/s10616-008-9152-1. Epub 2008 Jun 7.

DOI:10.1007/s10616-008-9152-1
PMID:19002854
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2553635/
Abstract

This paper reports for the first time, the Primary cell culture of hepatopancreas from edible crab Scylla serrata using crab saline, L-15 (Leibovitz), 1 x L-15 + crab saline, 2 x L-15 + crab saline, 3 x L-15 and citrate buffer without any serum. We could isolate and maintain E (Embryonalzellen), F (Fibrenzellen), B (Blasenzellen), R (Restzellen) and G (Granular cells). Upon seeding the hepatopancreatic E, F, B, and R cells showed different survival pattern over time than granular cells. A modified L-15 (3x) medium supported the best survival of hepatopancreatic E, F B, and R cells in in-vitro culture. However granular cells could be maintained for 184 days with L-15 (1x) + crab saline. Fetal bovine serum was not effective additive and hampered cell viability in present study.

摘要

本文首次报道了使用蟹盐水、L-15（Leibovitz）、1 x L-15 + 蟹盐水、2 x L-15 + 蟹盐水、3 x L-15 和柠檬酸盐缓冲液，在无任何血清的情况下从食用蟹锯缘青蟹（Scylla serrata）的肝胰腺中进行原代细胞培养。我们可以分离并维持 E（胚胎细胞）、F（纤维细胞）、B（囊泡细胞）、R（休止细胞）和 G（颗粒细胞）。在接种肝胰腺 E、F、B 和 R 细胞后，它们的存活模式随时间呈现出与颗粒细胞不同的特点。改良的 L-15（3x）培养基在体外培养中最有利于肝胰腺 E、F、B 和 R 细胞的存活。然而，颗粒细胞可以在 L-15（1x）+蟹盐水的条件下维持 184 天。在本研究中，胎牛血清不是有效的添加剂，反而会损害细胞活力。