Zilli L, Schiavone R, Scordella G, Zonno V, Verri T, Storelli C, Vilella S
Laboratory of General and Comparative Physiology, Department of Biological and Environmental Sciences and Technologies, University of Lecce, Via Provinciale Lecce-Monteroni, 73100 Lecce, Italy.
J Comp Physiol B. 2003 Jun;173(4):355-63. doi: 10.1007/s00360-003-0348-6. Epub 2003 May 8.
The goal of the present study was to evaluate the changes in the cell type composition and ATPase activities (total ATPase, ouabain-sensitive Na+/K(+)-ATPase, furosemide-sensitive Na(+)-ATPase) that occur during the different stages of the moulting cycle in the hepatopancreas of the Marsupenaeus japonicus. The results clearly suggest that the number of resorptive and fibrillar cell types changes significantly during the different stages. An inverse correlation between resorptive and fibrillar cells is observed during moulting (both in normally fed and fasted animals). Fasting, but not the moulting cycle, affects the number of blister-like cells. In the resorptive cells the enzymatic activities (total ATPases and ouabain-sensitive Na+/K(+)-ATPase) also change during the moulting in a cyclical manner. All these results are in agreement with and confirm the different functions carried out by the two cell types within the hepatopancreas.
本研究的目的是评估日本囊对虾肝胰腺在蜕皮周期不同阶段细胞类型组成和ATP酶活性（总ATP酶、哇巴因敏感的Na+/K(+)-ATP酶、速尿敏感的Na(+)-ATP酶）的变化。结果清楚地表明，吸收细胞和纤维状细胞的数量在不同阶段有显著变化。在蜕皮期间（正常喂食和禁食动物中均如此），观察到吸收细胞和纤维状细胞呈负相关。禁食而非蜕皮周期会影响泡状细胞的数量。在吸收细胞中，酶活性（总ATP酶和哇巴因敏感的Na+/K(+)-ATP酶）在蜕皮期间也呈周期性变化。所有这些结果与肝胰腺内两种细胞类型执行的不同功能一致并证实了这些功能。
