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Endogenous retrovirus particles and their repercussion effects on the growth behaviour of continuous hybridoma cultivation processes.内源性逆转录病毒颗粒及其对连续杂交瘤培养过程生长行为的影响。
Cytotechnology. 2001 Oct;37(2):83-92. doi: 10.1023/A:1019945416505.
2
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination.连续杂交瘤细胞培养中的生长行为影响：病毒污染的作用。
Cytotechnology. 1998 Nov;28(1-3):19-29. doi: 10.1023/A:1008000924426.
3
Evaluation of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus in mAb cell-culture.用于监测单克隆抗体细胞培养中逆转录病毒的定量产品增强型逆转录酶测定法的评估
Biologicals. 2002 Mar;30(1):15-26. doi: 10.1006/biol.2001.0290.
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Use of a quantitative product-enhanced reverse transcriptase assay to monitor retrovirus levels in mAb cell-culture and downstream processing.使用定量产品增强型逆转录酶测定法监测单克隆抗体细胞培养及下游加工过程中的逆转录病毒水平。
Biotechnol Prog. 2001 Jan-Feb;17(1):188-96. doi: 10.1021/bp000153q.
5
Re-examination of the McCoy cell line for confirmation of its mouse origin: karyotyping, electron microscopy and reverse transcriptase assay for endogenous retrovirus.重新检测 McCoy 细胞系以确认其小鼠起源：核型分析、电子显微镜检查及内源性逆转录病毒的逆转录酶测定。
Clin Diagn Virol. 1994 Apr;2(2):95-103. doi: 10.1016/0928-0197(94)90042-6.
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MuLV packaging systems as models for estimating/measuring retrovirus recombination frequency.作为估计/测量逆转录病毒重组频率模型的鼠白血病病毒包装系统
Dev Biol (Basel). 2001;106:169-79; discussion 253-63.
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Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells.
Hybridoma. 1994 Feb;13(1):69-76. doi: 10.1089/hyb.1994.13.69.
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Depression of reverse transcriptase activity by hybridoma supernatants: a potential problem in screening for retroviral contamination.
Biochem Biophys Res Commun. 1983 Apr 15;112(1):265-72. doi: 10.1016/0006-291x(83)91825-9.
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The reverse transcriptase activity in cell-free medium of chicken embryo fibroblast cultures is not associated with a replication-competent retrovirus.鸡胚成纤维细胞培养物的无细胞培养基中的逆转录酶活性与具有复制能力的逆转录病毒无关。
J Clin Virol. 1998 Jul 24;11(1):7-18. doi: 10.1016/s0928-0197(98)00042-7.
本文引用的文献
1
Hybridomas produce viruses as well as antibodies.杂交瘤既能产生病毒，也能产生抗体。
Immunol Today. 1982 Nov;3(11):292-4. doi: 10.1016/0167-5699(82)90028-7.
2
Serum-free growth medium for the cultivation of a wide spectrum of mammalian cells in stirred bioreactors.无血清生长培养基，用于搅拌式生物反应器中培养多种哺乳动物细胞。
Cytotechnology. 1988 Nov;1(4):319-29. doi: 10.1007/BF00365077.
3
Effects of dissolved oxygen levels and the role of extra- and intracellular amino acid concentrations upon the metabolism of mammalian cell lines during batch and continuous cultures.溶解氧水平的影响以及细胞内外氨基酸浓度在分批和连续培养过程中对哺乳动物细胞系代谢的作用。
Cytotechnology. 1998 May;26(3):185-97. doi: 10.1023/A:1007917409455.
4
Effects on growth behavior in continuous hybridoma cell cultures: The role of viral contamination.连续杂交瘤细胞培养中的生长行为影响：病毒污染的作用。
Cytotechnology. 1998 Nov;28(1-3):19-29. doi: 10.1023/A:1008000924426.
5
Micromorphology of murine tumor viruses and of affected cells.鼠肿瘤病毒及受感染细胞的微观形态学。
Fed Proc. 1962 Nov-Dec;21:936-41.
6
A sensitive method for the detection of murine C-type retroviruses.
J Virol Methods. 1997 Jan;63(1-2):155-65. doi: 10.1016/s0166-0934(96)02124-6.
7
Identification of amino acid residues critical for infection with ecotropic murine leukemia retrovirus.鉴定对嗜亲性鼠白血病逆转录病毒感染至关重要的氨基酸残基。
J Virol. 1993 Mar;67(3):1310-4. doi: 10.1128/JVI.67.3.1310-1314.1993.
8
Detection and characterization of murine ecotropic recombinant virus in myeloma and hybridoma cells.
Hybridoma. 1994 Feb;13(1):69-76. doi: 10.1089/hyb.1994.13.69.
9
Control of cationic amino acid transport and retroviral receptor functions in a membrane protein family.膜蛋白家族中阳离子氨基酸转运及逆转录病毒受体功能的调控
J Biol Chem. 1994 Jun 3;269(22):15445-50.
10
A critical site in the cell surface receptor for ecotropic murine retroviruses required for amino acid transport but not for viral reception.
Virology. 1994 Aug 1;202(2):1058-60. doi: 10.1006/viro.1994.1439.
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