Institute of Cell Culture Technology, Faculty of Technical Sciences, University of Bielefeld, D-33501, Bielefeld, Germany,
Cytotechnology. 2001 Oct;37(2):83-92. doi: 10.1023/A:1019945416505.
Hybridoma cells used for the production of monoclonal antibodies are also known to form growth inhibitory substances. Growth inhibitors already described in the literature belong to the class of peptides and proteins likeTGF-ss (Transforming Growth Factor-ss). The endogenous retrovirus particles - a further potential substance producing this kind of effect - are described here. To examine whether the retrovirus particles participated in growth inhibitory effects hybridoma cells were cultivated in continuous perfusion mode by using a special reactor set-up. A rapid increase of the signal in the supernatant which coincided with a decrease of viability could be observed by monitoring the reverse transcriptase-activity during this type of fermentation process. The examination of concentrated and fractionated supernatant from this period showed growth inhibitory effects in the biological assay (MTT-assay). Investigations of respective fractions demonstrated retrovirus particles with reverse transcriptase-activity. Based on RT-PCR data it was shown that only inhibitory fractions contain retrovirus particles which were of E-MuLV and MCF origin.
用于生产单克隆抗体的杂交瘤细胞也被发现会产生生长抑制物质。文献中已经描述的生长抑制剂属于 TGF-βs（转化生长因子-βs）等肽类和蛋白质。本文描述了另一种潜在的具有这种效应的物质——内源性逆转录病毒颗粒。为了研究逆转录病毒颗粒是否参与了生长抑制作用，将杂交瘤细胞在连续灌注模式下培养，使用特殊的反应器装置。在这种发酵过程中，通过监测逆转录酶活性，可以观察到上清液中的信号迅速增加，同时细胞活力下降。对该时期浓缩和分级的上清液的检查显示在生物测定（MTT 测定）中具有生长抑制作用。对各部分的研究表明，具有逆转录酶活性的逆转录病毒颗粒。基于 RT-PCR 数据表明，只有抑制性部分含有 E-MuLV 和 MCF 来源的逆转录病毒颗粒。
