原代培养猪冠状动脉：研究肝素对血管壁细胞增殖影响的模型。
Explants of porcine coronary artery in culture: A paradigm for studying the influence of heparin on vascular wall cell proliferation.
机构信息
Laboratoire de Biologie Cellulaire Expérimentale, UMR 6600, Université de Technologie de Compiègne, BP 20529, Compiègne, 60205, France,
出版信息
Cytotechnology. 2001 Sep;37(1):13-22. doi: 10.1023/A:1016195029359.
Explant cultures of porcine coronary artery provided a coculture model, used as a paradigm of arterial wall in contact with vascular prosthesis which allowed the study of spatial and temporal changes in cell phenotype. First cells emerging from the explant had an endothelial phenotype monitored by cytoimmunostaining. Percentages of anti-smooth muscle alpha-actin labelled cells were assessed at early and late phase by flow cytofluorometric analysis to control the effect of heparin. At 100 mug ml(-1), no effect on alpha-actin labelled cell growth has been detected. This result contrasted with the inhibition of monolayer cell cultures. At 500 mug ml(-1), the proliferation of smooth muscle cells was reduced. This explant system should be useful for testing drugs susceptible to interfere with restenosis.
猪冠状动脉的器官培养提供了一个共培养模型，用作与血管假体接触的动脉壁的范例，这允许研究细胞表型的时空变化。首先从器官中出现的细胞具有内皮细胞表型，通过细胞免疫染色监测。通过流式细胞荧光分析评估早期和晚期的抗平滑肌α-肌动蛋白标记细胞的百分比，以控制肝素的作用。在 100μg/ml（-1）时，未检测到对α-肌动蛋白标记细胞生长的影响。这一结果与单层细胞培养物的抑制作用形成对比。在 500μg/ml（-1）时，平滑肌细胞的增殖减少。该器官培养系统应可用于测试可能干扰再狭窄的药物。
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