Carere R G, Koo E W, Liu P P, Gotlieb A I
From the Vascular Research Laboratory, Department of Pathology, Banting and Best Diabetes Centre, and Department of Medicine, University of Toronto and The Toronto Hospital, Toronto, Ontario, Canada.
Cardiovasc Pathol. 1992 Apr-Jun;1(2):107-15. doi: 10.1016/1054-8807(92)90014-F.
The pathogenesis of coronary artery restenosis following angioplasty is not well understood. In order to carry out studies on the pathogenesis of restenosis, we developed and characterized an organ culture system using the porcine epicardial left anterior descending and circumflex coronary arteries. In nonangioplasty cultures at two weeks there was neointimal formation with intimal smooth muscle cells present within a loose matrix. All the neointimal cells beneath the endothelium stained positive for smooth muscle cell actin with α-SM1 antibody. Smooth muscle cell proliferation was maximum between four and eight days. Angioplasty, carried out by local balloon inflation at the onset of culture, resulted in an augmentation of the neointimal thickness and a further increase in smooth muscle cell proliferation. Endothelial cells, however, were absent from the angioplasty site, having been damaged and lost at the time of angioplasty. Because there is a response to angioplasty in this organ culture model, it will be a useful way of studying many of the factors likely to be important in the regulation of restenosis following angioplasty.
血管成形术后冠状动脉再狭窄的发病机制尚未完全清楚。为了开展再狭窄发病机制的研究，我们构建并描述了一种利用猪心外膜左前降支和回旋支冠状动脉的器官培养系统。在非血管成形术培养两周时，出现了新生内膜形成，内膜平滑肌细胞存在于疏松基质中。用α-SM1抗体检测，内皮细胞下方所有新生内膜细胞的平滑肌肌动蛋白染色均呈阳性。平滑肌细胞增殖在4至8天达到最大值。在培养开始时通过局部球囊扩张进行血管成形术，导致新生内膜厚度增加，平滑肌细胞增殖进一步加快。然而，血管成形术部位没有内皮细胞，它们在血管成形术时已受损并丢失。由于该器官培养模型对血管成形术有反应，它将成为研究血管成形术后再狭窄调节中许多可能重要因素的有用方法。
