Kobe Gakuin Women's College, 27-1 Hayashiyama-cho, 653-0861, Nagata-ku, Japan.
Cytotechnology. 2001 Nov;37(3):171-8. doi: 10.1023/A:1020580818979.
Menadione-catalyzed H(2)O(2) production by viable animal cells was proportional to the viable cell number, and H(2)O(2) production decreased with increasing cytotoxic effects after the incubation of cells with cytotoxic compounds. The cytotoxic effects of food additives, pesticides, antibiotics, heavy metals, phytotoxins, mycotoxins, and marine toxins were estimated using the above test employingNIH/3T3 and Neuro-2a cells. Synergistic effects of the toxin mixture were observed and acute cytotoxicity detected 1 h after the incubation of cells with toxins. This menadione-catalyzed H(2)O(2)production assay is rapid and simple compared to other popular cytotoxicity tests such as the MTT reduction assay and Neutral red inclusion test, requiring4 h. The menadione-catalyzed H(2)O(2) production assay is expected to be a useful food safety test for rapidly detecting toxic compounds having a basic cytotoxic effect on common animal cells.
用活细胞进行的 menadione 催化 H(2)O(2)生成与活细胞数量成正比，并且在用细胞毒性化合物孵育细胞后，随着细胞毒性作用的增加，H(2)O(2)生成减少。使用上述 NIH/3T3 和 Neuro-2a 细胞试验，估计食品添加剂、农药、抗生素、重金属、植物毒素、霉菌毒素和海洋毒素的细胞毒性作用。在细胞与毒素孵育 1 小时后，观察到毒素混合物的协同作用并检测到急性细胞毒性。与其他流行的细胞毒性试验（如 MTT 还原试验和中性红摄取试验）相比，这种 menadione 催化 H(2)O(2)生成试验快速且简单，所需时间为 4 小时。menadione 催化 H(2)O(2)生成试验有望成为一种有用的食品安全试验，用于快速检测对普通动物细胞具有基本细胞毒性作用的有毒化合物。
