The Prostate Stem Cell Laboratory, Institute of Cancer Research, 15 Cotdswold Rd, Sutton, Surrey, SM2 5NG, UK (E-mail,
Cytotechnology. 2003 Mar;41(2-3):189-96. doi: 10.1023/A:1024887009081.
The prostate gland is the site of the second most common cancer in men in the UK, with 9,280 deaths recorded in 2000. Another common disease of the prostate is benign prostatic hyperplasia and both conditions are believed to arise as a result of changes in the balance between cell proliferation and differentiation. There are three types of prostatic epithelial cell, proliferative basal, secretory luminal, and neuroendocrine. All three are believed to be derived from a common stem cell through differentiation along different pathways but the mechanisms behind these processes is poorly understood. In particular, there has until recently been very little information about prostate stem cell growth and differentiation. This review will discuss ways of distinguishing these prostate cell types using markers, such as keratins. Methods available for the culture of prostate epithelial cells and for the characterisation of stem cells both in monolayer and three-dimensional models are examined.
前列腺是英国男性中第二大常见癌症的发生部位，2000 年有 9280 人死亡。前列腺的另一种常见疾病是良性前列腺增生，这两种疾病都被认为是由于细胞增殖和分化之间平衡的改变而引起的。前列腺上皮细胞有三种类型，增殖基底细胞、分泌腔细胞和神经内分泌细胞。人们认为这三种细胞都起源于一个共同的干细胞，通过沿着不同的途径分化而来，但这些过程背后的机制还知之甚少。特别是，直到最近，关于前列腺干细胞的生长和分化的信息还非常有限。这篇综述将讨论使用角蛋白等标志物来区分这些前列腺细胞类型的方法。本文还研究了用于单层和三维模型培养前列腺上皮细胞和鉴定干细胞的方法。
