International Center for Biotechnology, Osaka University, 2-1, Yamada-oka, Suita, Osaka, 565-0871, Japan,
Cytotechnology. 2000 Mar;32(3):171-9. doi: 10.1023/A:1008171921282.
The effects of constant osmolarity, between 300 and500 mOsm/kg, on the metabolism of Chinese HamsterOvary (CHO) cells producing tissue plasminogenactivator (tPA) were compared between adhesion andsuspension cultures. In both suspension and adhesionculture, the specific rates of glucose consumption(nu(G)), lactate production (q(L)), and tPAproduction (q(tPA)) increased as osmolarityincreased, while these rates decreased when osmolaritywas higher than the respective critical levels. However, specific growth rate (mu) decreased withincrease in osmolarity and this slope grew steeper inthe osmolarity range higher than the critical level. The decrease in mu in the adhesion culture was morerapid than that in the suspension culture. Thecritical osmolarity for adhesion culture (400 mOsm/kg)was lower than that for suspension culture (450 mOsm/kg). These results indicated that the adhesionculture was more sensitive to increase of osmolaritythan the suspension culture, while the specific ratesobtained from the adhesion cultures were in general1.5- to 3-fold higher than those obtained from thesuspension cultures. Cell volume increased asosmolarity increased in both the suspension andadhesion cultures, as reported previously forsuspension culture of hybridoma cells, but there wasno morphological change in the suspension culture. Incontrast, cell height decreased and cell adhesion areamarkedly increased as osmolarity increased in theadhesion culture. This morphological change inadhesion cultures may be one reason for the highersensitivity of adherent cells to the increase ofosmolarity than suspended cells.
在 300 到 500mOsm/kg 的渗透压范围内，比较了贴壁和悬浮培养的中国仓鼠卵巢（CHO）细胞产生组织纤溶酶原激活剂（tPA）时，渗透压对代谢的影响。在悬浮和贴壁培养中，葡萄糖消耗速率（nu(G)）、乳酸生成速率（q(L)）和 tPA 生成速率（q(tPA)）均随渗透压的增加而增加，而当渗透压高于各自的临界水平时，这些速率则降低。然而，比生长速率（mu）随渗透压的增加而降低，而且在高于临界水平的渗透压范围内，斜率变得更陡。在贴壁培养中，mu 的下降速度比悬浮培养快。贴壁培养的临界渗透压（400mOsm/kg）低于悬浮培养（450mOsm/kg）。这些结果表明，与悬浮培养相比，贴壁培养对渗透压的增加更为敏感，而贴壁培养获得的比速率通常比悬浮培养高 1.5 到 3 倍。正如先前报道的杂交瘤细胞悬浮培养一样，在悬浮和贴壁培养中，细胞体积均随渗透压的增加而增加，但悬浮培养中没有形态变化。相比之下，在贴壁培养中，随着渗透压的增加，细胞高度降低，细胞黏附面积显著增加。这种贴壁培养中的形态变化可能是贴壁细胞比悬浮细胞对渗透压增加更敏感的原因之一。
