Department of Orthopedic Surgery, The Catholic University of Korea, College of Medicine, Seoul, Korea,
Cytotechnology. 2007 Jun;54(2):115-20. doi: 10.1007/s10616-007-9084-1. Epub 2007 Jun 23.
The osteogenic potential of autologous cultured osteoblasts mixed with fibrin when transplanted to bone defects was evaluated. Radial shaft defects over 15 mm were made in 30 New Zealand white rabbits. A total of 15 rabbits in the control group underwent an iliac bone graft and 15 rabbits in the experimental group underwent an autologous cultured osteoblast injection mixed with fibrin. Both groups were compared radiologically and 5 rabbits in each group were sacrificed for histological evaluation using H-E and Masson's trichrome stain at 3, 6, and 9 weeks. Osteogenesis in the control group progressed more rapidly than in the experimental group. However, at 9 weeks, bone formation in both groups were similar and showed no significant difference in terms of the amount of bone formation and the quality of bone union. Autologous cultured osteoblast transplantation mixed with fibrin in bone defects was found to produce bone efficiently.
评估了自体培养成骨细胞与纤维蛋白混合后移植到骨缺损部位的成骨潜力。在 30 只新西兰白兔的桡骨干缺损超过 15mm。对照组 15 只兔子行髂骨移植，实验组 15 只兔子行自体培养成骨细胞注射混合纤维蛋白。两组均进行影像学比较，每组 5 只兔子在 3、6 和 9 周时分别进行 H-E 和 Masson 三色染色的组织学评价。对照组的成骨过程比实验组更快。然而，在 9 周时，两组的骨形成相似，在骨形成量和骨愈合质量方面没有显著差异。在骨缺损中混合纤维蛋白的自体培养成骨细胞移植被发现能够有效地产生骨。
