Rudland P S, Hughes C M
Department of Biochemistry, University of Liverpool, United Kingdom.
J Cell Physiol. 1991 Feb;146(2):222-33. doi: 10.1002/jcp.1041460206.
Individual single-cell-cloned cell lines of the different rat mammary (Rama) cell types have been tested for their ability to bind the lectins Griffonia simplicifolia-1 (GS-1) and pokeweed mitogen (PWM) using fluorescent, histochemical, and radioactive assays. Myoepithelial-like cell lines isolated from neonatal rat mammary glands and from nonmetastasizing tumors strongly bind GS-1 and PWM, whereas the corresponding epithelial and fibroblastic cell lines do not. When the epithelial cell lines are grown on floating gels of polymerised rat tail collagen, the basally situated or peripheral cells are stained strongly with peroxidase-conjugated lectins, whereas the apically or luminally situated cells are unstained. The capacity of cell lines intermediate in morphology between epithelial and myoepithelial-like cells to bind to GS-1 is as follows: Rama 25 epithelial less than Rama 25-12 less than Rama 25-11 less than Rama 25-14 less than Rama 29 myoepithelial-like cells, the same order as for other markers of myoepithelial cells. Conjugated PWM, however, binds only to the myoepithelial-like cell lines. Treatment of Rama 25 epithelial cells with agents that disrupt microtubules accelerates their conversion to elongated, myoepithelial-like cells in culture. The binding of cells to GS-1 is observed prior to, and that to PWM after, the major morphological change. It is suggested that the stepwise appearances of carbohydrate receptors for GS-1 and PWM mark discrete stages in the differentiation of epithelial to myoepithelial-like cells in culture, in the same way that they mark similar differentiation stages in ductal development in mammary glands of prepubertal rats.
利用荧光、组织化学和放射性检测方法，对不同大鼠乳腺（Rama）细胞类型的单个单细胞克隆细胞系结合凝集素单叶豆凝集素-1（GS-1）和商陆有丝分裂原（PWM）的能力进行了检测。从新生大鼠乳腺和非转移性肿瘤中分离出的肌上皮样细胞系能强烈结合GS-1和PWM，而相应的上皮细胞系和成纤维细胞系则不能。当上皮细胞系在聚合的大鼠尾胶原漂浮凝胶上生长时，位于基底或周边的细胞被过氧化物酶偶联凝集素强烈染色，而位于顶端或管腔的细胞则未被染色。形态介于上皮细胞和肌上皮样细胞之间的细胞系结合GS-1的能力如下：Rama 25上皮细胞＜Rama 25-12＜Rama 25-11＜Rama 25-14＜Rama 29肌上皮样细胞，这与肌上皮细胞的其他标志物顺序相同。然而，偶联的PWM仅与肌上皮样细胞系结合。用破坏微管的试剂处理Rama 25上皮细胞可加速其在培养中转化为细长的肌上皮样细胞。在主要形态变化之前观察到细胞与GS-1的结合，而在形态变化之后观察到细胞与PWM的结合。有人提出，GS-1和PWM的碳水化合物受体的逐步出现标志着培养中上皮细胞向肌上皮样细胞分化的离散阶段，就像它们标志着青春期前大鼠乳腺导管发育中的类似分化阶段一样。
