Department of Gene Regulation and Differentiation, GBF-German Research Center for Biotechnology, Mascheroder Weg 1, Braunschweig, D-38124, Germany.
Cytotechnology. 2006 Mar;50(1-3):109-19. doi: 10.1007/s10616-006-9007-6. Epub 2006 May 20.
Many attempts have been undertaken to control transgene activity in mammalian cells. This is of importance for both applied biotechnology and basic research activities. State of the art regulatory systems use elements for transgene regulation which are unrelated to host regulatory networks and thus do not interfere with endogenous activities. Most of these regulation systems consist of transregulators and transregulator responding promoter elements that are derived from non mammalian origin. Apart from the tetracycline (Tet) regulated system which is most widely used for conditional gene expression at the moment, a number of new systems were created. These systems have been significantly refined and their performance makes them suitable for regulating transgenes not only in cellular systems but also in transgenic animals and for human therapeutic use.
人们已经尝试了许多方法来控制哺乳动物细胞中的转基因活性。这对于应用生物技术和基础研究活动都很重要。最先进的调控系统使用与宿主调控网络无关的转基因调控元件，因此不会干扰内源性活动。这些调控系统大多由来自非哺乳动物的转录调节剂和转录调节剂响应启动子元件组成。除了目前最广泛用于条件性基因表达的四环素（Tet）调控系统外，还创建了许多新的系统。这些系统已经得到了显著的改进，它们的性能使它们不仅适合于调控细胞系统中的转基因，也适合于转基因动物，并可用于人类治疗。
