Cytotechnology. 1999 Sep;31(1-2):185-93. doi: 10.1023/A:1008084508489.
Expression of mRNAs encoding ten laminin chain variants was studied by reverse transcription-polymerase chain reaction (RT-PCR) of mRNA extracted from cultured mouse blastocysts. alpha1, beta1 and gamma1 mRNAs, of which products form laminin-1, were the only chains expressed in blastocysts before hatching. alpha4 and alpha5 mRNAs were additionally expressed after attachment of blastocysts to culture dishes. alpha2 and beta2 mRNAs started to be expressed at the stage of allantois formation. Among ten laminin chains, only the mRNA encoding components of laminin-5 (alpha3, beta3 and gamma2) was missing in embryos up to the stage of allantois formation.
通过对培养的小鼠囊胚提取的 mRNA 进行逆转录-聚合酶链反应（RT-PCR），研究了编码十种层粘连蛋白链变体的 mRNAs 的表达。在孵化前的囊胚中，只有 alpha1、beta1 和 gamma1 mRNAs 的产物形成层粘连蛋白-1。alpha4 和 alpha5 mRNAs 在囊胚附着到培养皿后被额外表达。alpha2 和 beta2 mRNAs 在尿囊形成阶段开始表达。在十种层粘连蛋白链中，在胚胎发育到尿囊形成阶段之前，仅缺少编码层粘连蛋白-5（alpha3、beta3 和 gamma2）成分的 mRNA。
