不同癌症细胞系中糖基磷脂酰肌醇锚定癌胚抗原和碱性磷酸酶表达的差异模式。
Differential patterns of expression of glycosylphosphatidylinositol-anchored carcinoembryonic antigen and alkaline phosphatase in various cancer cell lines.
机构信息
Diagnostics R&D Department, Asahi Chemical Industry Co., Ltd., Ohito-cho, Shizuoka, 410-2321, Japan,
出版信息
Cytotechnology. 1999 Nov;31(3):255-63. doi: 10.1023/A:1008061424281.
The expression of glycosylphosphatidylinositol (GPI-anchored) carcinoembryonic antigen (CEA) and alkaline phosphatase (ALP) on the cell surface of various cancer cell lines and a lung diploid cell line (WI38) was investigated, with exposure of the cell lines to a cell differentiation agent (sodium butyrate) to induce cell differentiation and expression of the two tumor-associated antigens. In three colon (SW1222, SW1116, and HT-29) and stomach (MKN-45) cancer cell lines, all of which are double producers of CEA and ALP, the maximum expression of GPI-anchored CEA occurred with butyrate at a lower concentration than did that of GPI-anchored ALP. GPI-anchored ALP derived from colon (SW1222 and SW1116) and stomach (MKN-45 and MKN-1) cancer cell lines was heat-stable with and without exposure to butyrate, but GPI-anchored ALP derived from lung cancer cell lines (PC-6, PC13, PC-14, WI26VA4, and WI38VA13) showed a variety of heat stabilities, depending on cell line, butyrate exposure, and SV40 transformation.
研究了糖基磷脂酰肌醇（GPI）锚定癌胚抗原（CEA）和碱性磷酸酶（ALP）在各种癌细胞系和肺二倍体细胞系（WI38）表面的表达情况，并通过细胞分化剂（丁酸钠）暴露于细胞系，以诱导细胞分化和两种肿瘤相关抗原的表达。在三种结肠（SW1222、SW1116 和 HT-29）和胃（MKN-45）癌细胞系中，CEA 和 ALP 均为双产物，GPI 锚定 CEA 的最大表达发生在较低浓度的丁酸钠下，而 GPI 锚定 ALP 的表达则较低。来自结肠（SW1222 和 SW1116）和胃（MKN-45 和 MKN-1）癌细胞系的 GPI 锚定 ALP 在有或没有丁酸钠暴露的情况下均具有热稳定性，但来自肺癌细胞系（PC-6、PC13、PC-14、WI26VA4 和 WI38VA13）的 GPI 锚定 ALP 表现出多种热稳定性，这取决于细胞系、丁酸钠暴露和 SV40 转化。
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