Departament of Biotechnological Processes, School of Chemical Engineering, State University of Campinas, P.O. Box 6066, 13083-852, Campinas, SP, Brazil.
Cytotechnology. 2008 May;57(1):73-81. doi: 10.1007/s10616-008-9139-y. Epub 2008 Mar 2.
Drosophila melanogaster Schneider 2 (S2) cells have been increasingly used as a suitable expression system for the production of different recombinant proteins, and the employment of bioreactors for large-scale culture is an important tool for this purpose. In this work, Drosophila S2 cells producing the rabies virus glycoprotein RVGP were cultivated in bioreactor, employing a serum-free medium, aiming an improvement in cell growth and in glycoprotein production. To overcome cell growth limitation commonly observed in stirred flasks, different experiments in bioreactor were performed, in which some system modifications were carried out to attain the desired goal. The study showed that this cell line is considerably sensitive to hydrodynamic forces, and a high cell density (about 16.0 x 10(6) cells mL(-1)) was only obtained when Pluronic F68((R)) percentage was increased to 0.6% (w/v). Despite ammonium concentration affected RVGP production, and also cell growth, an elevated amount of the target protein was obtained, attaining 563 ng 10(-7) cells.
黑腹果蝇 Schneider 2（S2）细胞已被越来越多地用作生产不同重组蛋白的合适表达系统，而生物反应器的大规模培养是实现这一目的的重要工具。在这项工作中，生产狂犬病病毒糖蛋白 RVGP 的果蝇 S2 细胞在生物反应器中使用无血清培养基进行培养，旨在提高细胞生长和糖蛋白产量。为了克服在搅拌瓶中常见的细胞生长限制，在生物反应器中进行了不同的实验，其中进行了一些系统修改以达到预期的目标。研究表明，该细胞系对流体动力非常敏感，只有当 Pluronic F68((R))的百分比增加到 0.6%（w/v）时，才能获得高密度（约 16.0 x 10(6) 个细胞 mL(-1)）。尽管铵浓度会影响 RVGP 的产生和细胞生长，但仍获得了大量的目标蛋白，达到了 563 ng 10(-7) 个细胞。
