Ross Eye Institute, Department of Ophthalmology, University at Buffalo, 14214 , NY, USA,
Cytotechnology. 2004 Jul;45(3):155-60. doi: 10.1007/s10616-004-7256-9.
Exclusion of Hoechst 33342 dye is a characteristic common to stem cells, as well as chemotherapy-resistant cancer cells. Normally, these dye-excluding cells can be sorted from enzymatically dissociated tissues with a UV cell sorter/flow cytometer. UV-flow cytometry can be expensive, time-consuming and not readily available to all laboratories. We have developed a simple, high-throughput 96-well microtiter plate assay by which cell populations can be quickly screened for Hoechst dye uptake and exclusion. The method is compatible with green-fluorescent EGFP expressing cells, often used in stem cell biology. Useful applications for this assay will be the rapid screening of clonal stem cell populations and tumor cells for Hoechst dye uptake.
排除 Hoechst 33342 染料是干细胞以及化疗耐药癌细胞的共同特征。通常，这些排斥染料的细胞可以用紫外线细胞分选器/流式细胞仪从酶解组织中分离出来。紫外线流式细胞术可能昂贵、耗时且并非所有实验室都能轻易获得。我们开发了一种简单、高通量的 96 孔微量滴定板测定法，可以快速筛选细胞群体对 Hoechst 染料的摄取和排除。该方法与常用于干细胞生物学的绿色荧光 EGFP 表达细胞兼容。该测定法的有用应用将是快速筛选克隆干细胞群体和肿瘤细胞对 Hoechst 染料的摄取。
