Laboratory of Growth Regulators, Institute of Experimental Botany, Academy of Sciences of the Czech Republic, 10227, Praha 10, Czech Republic,
Cytotechnology. 2001 Jul;36(1-3):117-23. doi: 10.1023/A:1014020415912.
An analog of aromatic cytokinins, the 2,6,9-trisubstituted purine derivative bohemine, was applied to cultures of mouse hybridoma cells in order to analyze its capacity of suppressing cell growth and maintaining or enhancing the production of monoclonal antibody. Addition of bohemine at concentrations in the range of1-10 muM resulted in a short-term arrest of growth and of monoclonal antibody production. The short-term suppression of cell functions was followed by a significant temporary increase of specific growth rate and of specific production rate. The steady-state viable cell density values, found in semicontinuous cultures, showed a certain stimulation of cell growth in the range of micromolar concentrations of bohemine, and inhibition of growth at 10 and 30 muM concentrations. The profiles of cell cycle phases indicated that hybridoma cells are retarded both at the G(1)/S boundary and at the G(2)/M boundary, depending on bohemine concentration. The existence of the sequence of events,from suppression to stimulation, suggests that bohemine probably modulates more than one regulatory pathway in the cell.
一种芳香族细胞分裂素类似物，2,6,9-三取代嘌呤衍生物 bohimine，被应用于小鼠杂交瘤细胞的培养中，以分析其抑制细胞生长和维持或增强单克隆抗体产生的能力。在 1-10 μM 的浓度范围内添加 bohimine 会导致细胞生长和单克隆抗体产生的短期停滞。细胞功能的短期抑制之后，特定生长速率和特定产生速率会显著暂时增加。在半连续培养中发现的稳定态活细胞密度值在 bohimine 的微摩尔浓度范围内显示出对细胞生长的一定刺激作用，而在 10 和 30 μM 浓度下则抑制生长。细胞周期各阶段的分布表明，杂交瘤细胞在 bohimine 浓度的影响下，在 G1/S 边界和 G2/M 边界均受到阻滞。从抑制到刺激的一系列事件的存在表明，bohimine 可能在细胞中调节了不止一条调节途径。
