Hayter P M, Kirkby N F, Spier R E
Biological Laboratory, University of Kent, Canterbury, UK.
Enzyme Microb Technol. 1992 Jun;14(6):454-61. doi: 10.1016/0141-0229(92)90137-d.
Factors affecting cell growth and antibody production in a mouse hybridoma were investigated. Antibody was produced during the growth and decline phases of a batch culture with an increase in the specific rate of antibody production during the decline phase. The specific rate of antibody production was also increased in cells arrested by 2 mM thymidine, suggesting that cell proliferation and antibody production can be uncoupled. Reduced serum concentrations resulted in lower cell growth rates but increased antibody production rates. However, this trend was reversed in hybridomas which had been arrested by thymidine, since the highest antibody production rate was associated with high serum concentrations. Likewise, in proliferating cells, the optimum pH for antibody production (pH 6.8) was lower than the optimum pH for cell growth (pH 7.2), whereas in thymidine-blocked cells, the highest antibody production rate was at pH 7.2. High antibody production rates and product yields were also associated with low growth rates in continuous cultures. The possibility that antibody was under cell cycle control was investigated in synchronized hybridoma cultures. Antibody production occurred during G1 and G2 with a decline in the M phase and evidence of a further decline in the S phase. Thus antibody production was not restricted to the G1 and S phase in this hybridoma.
研究了影响小鼠杂交瘤细胞生长和抗体产生的因素。在分批培养的生长和衰退阶段均产生抗体，衰退阶段抗体产生的比速率增加。用2 mM胸苷阻滞的细胞中抗体产生的比速率也增加，这表明细胞增殖和抗体产生可以解偶联。血清浓度降低导致细胞生长速率降低，但抗体产生速率增加。然而，在被胸苷阻滞的杂交瘤中这种趋势相反，因为最高的抗体产生速率与高血清浓度相关。同样，在增殖细胞中，抗体产生的最适pH（pH 6.8）低于细胞生长的最适pH（pH 7.2），而在胸苷阻滞的细胞中，最高的抗体产生速率出现在pH 7.2时。在连续培养中，高抗体产生速率和产物产量也与低生长速率相关。在同步化的杂交瘤培养物中研究了抗体受细胞周期调控的可能性。抗体产生发生在G1期和G2期，M期下降，S期有进一步下降的迹象。因此，在这种杂交瘤中抗体产生并不局限于G1期和S期。
