Institute of Agricultural and Forest Engineering, University of Tsukuba, Tennodai 1-1-1, Tsukuba, Ibaraki, 305-8572, Japan,
Cytotechnology. 2001 Jul;36(1-3):155-61. doi: 10.1023/A:1014053306343.
The influence of capsaicin processing on human intestinal cell line Caco-2 was examined by measuring transepithelial electrical resistance (TER). There was an increase in permeability at high concentration (200 to 500 muM) of capsaicin, and the effect was inhibited by pretreatment of capsazepine, which is a competitive antagonist of the vanilloid receptor 1 (VR1). LDH-activity as well as changes in intracellular Ca(2+) were determined to know whether or not capsaicin affected TER activity through its influence on the tight junction. We also determined the expression of the VR1-like protein on Caco-2 cells in time-dependent manner by western blotting using vanilloid receptor (VR1) antiserum. Our results showed that the permeability increase by capsaicin was through binding to VR1-like protein of Caco-2 cells.
通过测量跨上皮电阻(TER)来研究辣椒素处理对人肠道细胞系 Caco-2 的影响。在高浓度(200 至 500 μM)辣椒素存在的情况下,通透性增加,而辣椒素的预处理(辣椒素的竞争性拮抗剂)抑制了该作用,辣椒素的预处理(辣椒素的竞争性拮抗剂)抑制了该作用,辣椒素的预处理(辣椒素的竞争性拮抗剂)抑制了该作用。用辣椒素处理还会导致细胞内 Ca(2+)的变化,从而影响紧密连接。我们还通过使用香草醛受体(VR1)抗血清进行 Western 印迹,以时间依赖性的方式确定 Caco-2 细胞上 VR1 样蛋白的表达。我们的结果表明,辣椒素引起的通透性增加是通过与 Caco-2 细胞上的 VR1 样蛋白结合实现的。