Department of Laboratory Medicine, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Molecules. 2023 Jul 6;28(13):5258. doi: 10.3390/molecules28135258.
Obesity represents a major health challenge because it substantially increases the risk of metabolic diseases. Capsaicin, the major active ingredient of spp., has been reported to possess anti-obesity activity. Hereon, the effect of capsaicin on glucose uptake and consumption in hepatocytes was extensively studied. Capsaicin was shown to accelerate the glucose uptake/consumption and the ATP production of hepatocytes. The elevation of intracellular Ca was thought to be a potential mechanism. By transcriptome analysis, 78, 146 and 507 differentially expressed genes (DEGs) were identified between capsaicin and the control group for 4 h, 12 h and 24 h treatments. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed that most of the DEGs were involved in canonical pathways, like MAPK and PI3K-AKT signaling pathways. Clustering analysis showed that many DEGs were associated with glucose and amino acid metabolism. The variation trend in genes related to glucose and amino acid metabolism (like CTH, VEGFA, PCK2 and IGFBP3) in the quantitative PCR (q-PCR) assay was consistent with the transcriptome data. These results demonstrated that capsaicin efficiently accelerated the glucose uptake and consumption of hepatocytes.
肥胖是一个主要的健康挑战，因为它大大增加了患代谢疾病的风险。辣椒素， spp. 的主要活性成分，据报道具有抗肥胖活性。在此基础上，我们广泛研究了辣椒素对肝细胞葡萄糖摄取和消耗的影响。结果表明，辣椒素能加速肝细胞的葡萄糖摄取/消耗和 ATP 产生。细胞内 Ca 的升高被认为是一种潜在的机制。通过转录组分析，在辣椒素处理 4 小时、12 小时和 24 小时后，与对照组相比，分别有 78、146 和 507 个差异表达基因（DEGs）被鉴定出来。京都基因与基因组百科全书（KEGG）分析表明，大多数 DEGs 参与了经典途径，如 MAPK 和 PI3K-AKT 信号通路。聚类分析表明，许多 DEGs 与葡萄糖和氨基酸代谢有关。定量 PCR（q-PCR）检测中与葡萄糖和氨基酸代谢相关的基因（如 CTH、VEGFA、PCK2 和 IGFBP3）的变化趋势与转录组数据一致。这些结果表明，辣椒素能有效地加速肝细胞的葡萄糖摄取和消耗。
