Animal Cell Technology Group, School of Chemical Engineering, University of Birmingham, Birmingham, B15 2TT, UK.
Cytotechnology. 1999 Jul;30(1-3):59-69. doi: 10.1023/A:1008055904642.
The importance of cell cycle analysis in cell culture development has been widely recognised. Whether such analysis is useful in indicating future performance of high cell density culture is uncertain. Using flow cytometric approach to address this question, we utilised the fraction of cells in the S phase to control specific growth rate and productivity in spin filter perfusion cultures and found a significant increase in the accumulated interferon-gamma over that obtained from the nutrient-based controlled fed culture. While a general decrease with time exists in both percentage of S phase cells and specific growth rate, a clear oscillatory behaviour of both parameters is found in perfusion cultures.
细胞周期分析在细胞培养开发中的重要性已得到广泛认可。但这种分析是否有助于预测高密度培养的未来表现尚不确定。采用流式细胞术方法来解决这个问题，我们利用 S 期细胞的比例来控制旋转滤器灌注培养中的特定生长速率和生产力，并发现干扰素-γ的积累量显著高于基于营养物的控制补料培养。尽管 S 期细胞的百分比和特定生长速率随时间普遍下降，但在灌注培养中发现这两个参数都存在明显的振荡行为。
