Emery A N, Jan D C, al-Rubeai M
BBSRC Centre for Biochemical Engineering, School of Chemical Engineering, University of Birmingham, Edgbaston, UK.
Appl Microbiol Biotechnol. 1995 Nov;43(6):1028-33. doi: 10.1007/BF00166920.
The abilities of various methods of oxygenation to meet the demands of high-cell-density culture were investigated using a spin filter perfusion system in a bench-top bioreactor. Oxygen demand at high cell density could not be met by sparging with air inside a spin filter (oxygen transfer values in this condition were comparable with those for surface aeration). Sparging with air outside a spin filter gave adequate oxygen transfer for the support of cell concentrations above 10(7) ml-1 in fully aerobic conditions but the addition of antifoam to control foaming caused blockage of the spinfilter mesh. Bubble-free aeration through immersed silicone tubing with pure oxygen gave similar oxygen transfer rates to that of sparging with air but without the problems of bubble damage and fouling of the spin filter. A supra-optimal level of dissolved oxygen (478% air saturation) inhibited cell growth. However, cells could recover from this stress and reach high density after reduction of the dissolved oxygen level to 50% air saturation.
使用台式生物反应器中的旋转过滤器灌注系统，研究了各种氧合方法满足高细胞密度培养需求的能力。在旋转过滤器内部用空气鼓泡无法满足高细胞密度下的氧气需求（在此条件下的氧传递值与表面曝气的氧传递值相当）。在旋转过滤器外部用空气鼓泡在完全有氧条件下能提供足够的氧传递，以支持细胞浓度高于10(7) ml-1，但添加消泡剂以控制泡沫会导致旋转过滤器滤网堵塞。通过浸入式硅胶管通入纯氧进行无泡曝气，其氧传递速率与用空气鼓泡相似，但不存在气泡损伤和旋转过滤器污染的问题。超最佳水平的溶解氧（478%空气饱和度）会抑制细胞生长。然而，将溶解氧水平降至50%空气饱和度后，细胞可以从这种应激中恢复并达到高密度。
