The Institute of Physical and Chemical Research (RIKEN), RIKEN Cell Bank, 3-1-1 Koyadai, Tsukuba Science City, Ibaraki, 305-0074, Japan.
Cytotechnology. 2000 Oct;34(1-2):101-10. doi: 10.1023/A:1008156921001.
When CD4(+) T cell-rich population appears in theinitial trial in induction cultures of humanautologous cytotoxic T lymphocytes (CTL), the cultureresults frequently in no or weak killing activity andtherefore usually be discarded as an ;unsuccessful'CTL induction culture. However, addition of theinitial CD4(+) T cell-rich population enabledefficient induction of the autologous CTL in theensuing trials. The CTL thus generated exhibitedstronger killing activities against autologous braintumor cells and ovarian tumor cells than previouslyobserved. This simple recycling of the primed butinert CD4(+) T cell-rich population for CTLinduction will promote clinical practice of adoptiveimmunotherapy of human tumors with autologous CTL.
当初始诱导培养的人源细胞毒性 T 淋巴细胞（CTL）中出现 CD4(+)T 细胞丰富群体时，培养结果通常为无或弱杀伤活性，因此通常被丢弃为“不成功”的 CTL 诱导培养。然而，添加初始 CD4(+)T 细胞丰富群体可在随后的试验中有效地诱导自体 CTL。由此产生的 CTL 对自体脑肿瘤细胞和卵巢肿瘤细胞的杀伤活性比以前观察到的更强。这种简单地回收已被激活但处于惰性状态的富含 CD4(+)T 细胞的群体用于 CTL 诱导，将促进用自体 CTL 进行人类肿瘤过继免疫治疗的临床实践。
