一种高效的无血清、悬浮细胞培养工艺，用于生产基因治疗用重组腺病毒载体。
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.
机构信息
Fermentation and Cell Culture Development, Biotechnology R&D (CMC), Berlex Biosciences, 15409 San Pablo Avenue, Richmond, CA, 94804, U.S.A.
出版信息
Cytotechnology. 1998 Nov;28(1-3):81-9. doi: 10.1023/A:1008021428969.
Abstract
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.
摘要
我们开发了一种高效、可重复且可扩展的细胞培养工艺，用于培养表达治疗性转基因的重组腺病毒载体，以进行临床试验。支持 E1 缺失型腺病毒复制的 HEK 293 细胞最初被适应于无血清培养基和悬浮培养。随后的研究集中在悬浮培养生物反应器中的感染、病毒生产和收获上。未来的研究计划解决 HEK 293 以及其他细胞系中腺病毒生产的动力学问题。
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