一种高效的无血清、悬浮细胞培养工艺，用于生产基因治疗用重组腺病毒载体。
A high-yielding serum-free, suspension cell culture process to manufacture recombinant adenoviral vectors for gene therapy.
机构信息
Fermentation and Cell Culture Development, Biotechnology R&D (CMC), Berlex Biosciences, 15409 San Pablo Avenue, Richmond, CA, 94804, U.S.A.
出版信息
Cytotechnology. 1998 Nov;28(1-3):81-9. doi: 10.1023/A:1008021428969.
Abstract
We have developed an efficient, reproducible, and scaleable cell culture process for a recombinant adenoviral vector expressing therapeutic transgenes for clinical trials. HEK 293 cells - which support the propagation of E1 deficient adenovirus - were first adapted to serum free media and suspension growth. Subsequent studies focused on the infection, virus production and harvest from suspension culture bioreactors. Future studies are planned to address the kinetics of adenovirus production in HEK 293 as well as in other cell lines.
摘要
我们开发了一种高效、可重复且可扩展的细胞培养工艺，用于培养表达治疗性转基因的重组腺病毒载体，以进行临床试验。支持 E1 缺失型腺病毒复制的 HEK 293 细胞最初被适应于无血清培养基和悬浮培养。随后的研究集中在悬浮培养生物反应器中的感染、病毒生产和收获上。未来的研究计划解决 HEK 293 以及其他细胞系中腺病毒生产的动力学问题。
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本文引用的文献
1
Manipulation of adenovirus vectors.腺病毒载体的操控
Methods Mol Biol. 1991;7:109-28. doi: 10.1385/0-89603-178-0:109.
2
Improvement of recombinant protein production with the human adenovirus/293S expression system using fed-batch strategies.采用补料分批策略，利用人腺病毒/293S表达系统提高重组蛋白产量。
Biotechnol Bioeng. 1996 Sep 20;51(6):613-23. doi: 10.1002/(SICI)1097-0290(19960920)51:6<613::AID-BIT1>3.0.CO;2-K.
3
The TMC Worldwide Gene Therapy Enrollment Report, end 1996.1996年末的TMC全球基因治疗入组报告。
Hum Gene Ther. 1997 Apr 10;8(6):775-800. doi: 10.1089/hum.1997.8.6-775.
4
Analytical anion-exchange HPLC of recombinant type-5 adenoviral particles.重组5型腺病毒颗粒的分析型阴离子交换高效液相色谱法。
Hum Gene Ther. 1997 Mar 1;8(4):453-65. doi: 10.1089/hum.1997.8.4-453.
5
Scale-up of the adenovirus expression system for the production of recombinant protein in human 293S cells.用于在人293S细胞中生产重组蛋白的腺病毒表达系统的放大。
Cytotechnology. 1994;15(1-3):145-55. doi: 10.1007/BF00762389.
6
Transfer of genes to humans: early lessons and obstacles to success.基因向人类的转移：早期经验教训与成功面临的障碍
Science. 1995 Oct 20;270(5235):404-10. doi: 10.1126/science.270.5235.404.
7
Accuracy of the endpoint assay for virus titration.病毒滴定终点测定法的准确性。
Cytotechnology. 1992;8(3):231-6. doi: 10.1007/BF02522040.
8
Human adenoviruses: growth, purification, and transfection assay.人腺病毒：生长、纯化及转染测定
Methods Enzymol. 1979;58:425-35. doi: 10.1016/s0076-6879(79)58157-9.
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