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Generation of novel chimeric LacdiNAcS by gene fusion of alpha-lactalbumin and beta1,4-galactosyltransferase 1.

作者信息

Do Su-Il

机构信息

Department of Life Science, Ajou University, Suwon City, Republic of Korea.

出版信息

Glycoconj J. 2009 Jul;26(5):567-75. doi: 10.1007/s10719-008-9208-7. Epub 2008 Nov 12.

DOI:10.1007/s10719-008-9208-7
PMID:19003527
Abstract

Novel chimeric lacdiNAc (GalNAc(beta1-4)GlcNAc) synthase (c-LacdiNAcS) was generated by gene fusion of alpha-lactalbumin (alpha-LA) and beta1,4-galactosyltransferase 1 (beta1,4-GalT1). c-LacdiNAcS was expressed in Lec8 Chinese hamster ovary (Lec8 CHO) cells and exhibited N-acetylgalactosaminyltransferase (GalNAcT) activity in the absence of exogenous alpha-LA as well as other glycosyltransferase activities including lactose synthase (LacS), and beta1,4-GalT. These glycosyltransferase activities of c-LacdiNAcS were compared to those activities induced in LacS system under the co-presence of bovine beta1,4-GalT1 and alpha-LA, indicating that each domain of alpha-LA and beta1,4-GalT1 on c-LacdiNAcS is not only folding correctly, but also interacting together. Furthermore, c-LacdiNAcS was found to be auto-lacdiNAcylated and can synthesize lacdiNAc structures on cellular glycoproteins, demonstrating that GalNAcT activity of c-LacdiNAcS is functional in Lec8 CHO cells.

摘要

相似文献

1
Generation of novel chimeric LacdiNAcS by gene fusion of alpha-lactalbumin and beta1,4-galactosyltransferase 1.
Glycoconj J. 2009 Jul;26(5):567-75. doi: 10.1007/s10719-008-9208-7. Epub 2008 Nov 12.
2
Bovine mammary gland UDP-GalNAc:GlcNAcbeta-R beta1-->4-N-acetylgalactosaminyltransferase is glycoprotein hormone nonspecific and shows interaction with alpha-lactalbumin.牛乳腺UDP-N-乙酰半乳糖胺：β-1,4-N-乙酰半乳糖胺基转移酶对糖蛋白激素不具有特异性，并显示出与α-乳白蛋白的相互作用。
Glycobiology. 1999 Feb;9(2):115-23. doi: 10.1093/glycob/9.2.115.
3
Novel poly-GalNAcbeta1-4GlcNAc (LacdiNAc) and fucosylated poly-LacdiNAc N-glycans from mammalian cells expressing beta1,4-N-acetylgalactosaminyltransferase and alpha1,3-fucosyltransferase.来自表达β1,4-N-乙酰半乳糖胺基转移酶和α1,3-岩藻糖基转移酶的哺乳动物细胞的新型聚-GalNAcbeta1-4GlcNAc（LacdiNAc）和岩藻糖基化聚-LacdiNAc N-聚糖。
J Biol Chem. 2005 Apr 1;280(13):12810-9. doi: 10.1074/jbc.M414273200. Epub 2005 Jan 14.
4
Novel induction of alpha-lactalbumin-mediated lacdiNAc-R expression in vivo.体内新型诱导α-乳白蛋白介导的乳糖二胺聚糖-R表达
Biochem J. 2000 May 15;348 Pt 1(Pt 1):229-34.
5
Alpha-lactalbumin affects the acceptor specificity of Lymnaea stagnalis albumen gland UDP-GalNAc:GlcNAc beta-R beta 1-->4-N-acetylgalactosaminyltransferase: synthesis of GalNAc beta 1-->4Glc.α-乳白蛋白影响椎实螺白蛋白腺UDP-N-乙酰半乳糖胺：N-乙酰葡糖胺β-Rβ1→4-N-乙酰半乳糖胺基转移酶的受体特异性：GalNAcβ1→4Glc的合成。
Proc Natl Acad Sci U S A. 1996 Sep 17;93(19):10111-6. doi: 10.1073/pnas.93.19.10111.
6
Differential expression of LacdiNAc sequences (GalNAc beta 1-4GlcNAc-R) in glycoproteins synthesized by Chinese hamster ovary and human 293 cells.中国仓鼠卵巢细胞和人293细胞合成的糖蛋白中LacdiNAc序列（GalNAcβ1-4GlcNAc-R）的差异表达。
Glycobiology. 1997 Mar;7(2):183-94. doi: 10.1093/glycob/7.2.183.
7
Effect of hyperthermia on activity of three glycosyltransferases in Chinese hamster ovary cells.热疗对中国仓鼠卵巢细胞中三种糖基转移酶活性的影响。
Cancer Res. 1988 Oct 15;48(20):5717-21.
8
Differential effect of alpha-lactalbumin on beta-1,4-galactosyltransferase IV activities.α-乳白蛋白对β-1,4-半乳糖基转移酶IV活性的差异影响。
Biochem Biophys Res Commun. 1998 Mar 27;244(3):637-41. doi: 10.1006/bbrc.1998.8327.
9
Lactose synthase components in milk: concentrations of alpha-lactalbumin and beta1,4-galactosyltransferase in milk of cows from several breeds at various stages of lactation.牛奶中的乳糖合酶成分：不同泌乳阶段几个品种奶牛乳汁中α-乳白蛋白和β1,4-半乳糖基转移酶的浓度。
Reprod Domest Anim. 2009 Apr;44(2):241-7. doi: 10.1111/j.1439-0531.2007.01047.x. Epub 2008 Aug 6.
10
Glycan structure and serum half-life of recombinant CTLA4Ig, an immunosuppressive agent, expressed in suspension-cultured rice cells with coexpression of human β1,4-galactosyltransferase and human CTLA4Ig.在悬浮培养的水稻细胞中共表达人β1,4-半乳糖基转移酶和人CTLA4Ig时表达的免疫抑制剂重组CTLA4Ig的聚糖结构和血清半衰期。
Glycoconj J. 2015 May;32(3-4):161-72. doi: 10.1007/s10719-015-9590-x. Epub 2015 May 14.

本文引用的文献

1
Apical Golgi localization of N,N'-diacetyllactosediamine synthase, beta4GalNAc-T3, is responsible for LacdiNAc expression on gastric mucosa.N，N'-二乙酰乳糖二胺合酶β4GalNAc-T3的高尔基体顶端定位负责胃黏膜上乳糖二胺（LacdiNAc）的表达。
Glycobiology. 2006 Sep;16(9):777-85. doi: 10.1093/glycob/cwl005. Epub 2006 May 25.
2
Novel poly-GalNAcbeta1-4GlcNAc (LacdiNAc) and fucosylated poly-LacdiNAc N-glycans from mammalian cells expressing beta1,4-N-acetylgalactosaminyltransferase and alpha1,3-fucosyltransferase.来自表达β1,4-N-乙酰半乳糖胺基转移酶和α1,3-岩藻糖基转移酶的哺乳动物细胞的新型聚-GalNAcbeta1-4GlcNAc（LacdiNAc）和岩藻糖基化聚-LacdiNAc N-聚糖。
J Biol Chem. 2005 Apr 1;280(13):12810-9. doi: 10.1074/jbc.M414273200. Epub 2005 Jan 14.
3
LacdiNAc-glycans constitute a parasite pattern for galectin-3-mediated immune recognition.乳糖胺聚糖构成了半乳糖凝集素-3介导的免疫识别的一种寄生虫模式。
J Immunol. 2004 Aug 1;173(3):1902-7. doi: 10.4049/jimmunol.173.3.1902.
4
Antigenic glycans in parasitic infections: implications for vaccines and diagnostics.寄生虫感染中的抗原聚糖：对疫苗和诊断的影响。
Arch Biochem Biophys. 2004 Jun 15;426(2):182-200. doi: 10.1016/j.abb.2004.04.004.
5
Molecular cloning and characterization of a novel human beta 1,4-N-acetylgalactosaminyltransferase, beta 4GalNAc-T3, responsible for the synthesis of N,N'-diacetyllactosediamine, galNAc beta 1-4GlcNAc.一种新型人类β1,4-N-乙酰半乳糖胺基转移酶β4GalNAc-T3的分子克隆与特性分析，该酶负责合成N，N'-二乙酰乳糖二胺，即GalNAcβ1-4GlcNAc。
J Biol Chem. 2003 Nov 28;278(48):47534-44. doi: 10.1074/jbc.M308857200. Epub 2003 Sep 9.
6
Molecular cloning and enzymatic characterization of a UDP-GalNAc:GlcNAc(beta)-R beta1,4-N-acetylgalactosaminyltransferase from Caenorhabditis elegans.秀丽隐杆线虫UDP-N-乙酰半乳糖胺：N-乙酰葡糖胺（β）-Rβ1,4-N-乙酰半乳糖胺基转移酶的分子克隆与酶学特性分析
J Biol Chem. 2002 Sep 20;277(38):34924-32. doi: 10.1074/jbc.M206112200. Epub 2002 Jul 11.
7
alpha-Lactalbumin (LA) stimulates milk beta-1,4-galactosyltransferase I (beta 4Gal-T1) to transfer glucose from UDP-glucose to N-acetylglucosamine. Crystal structure of beta 4Gal-T1 x LA complex with UDP-Glc.α-乳白蛋白（LA）刺激乳β-1,4-半乳糖基转移酶I（β4Gal-T1）将UDP-葡萄糖中的葡萄糖转移至N-乙酰葡糖胺。β4Gal-T1与LA复合物及UDP-Glc的晶体结构。
J Biol Chem. 2001 Oct 5;276(40):37665-71. doi: 10.1074/jbc.M102458200. Epub 2001 Aug 2.
8
Point mutations identified in Lec8 Chinese hamster ovary glycosylation mutants that inactivate both the UDP-galactose and CMP-sialic acid transporters.在Lec8中国仓鼠卵巢糖基化突变体中鉴定出的点突变，这些突变使UDP-半乳糖和CMP-唾液酸转运蛋白均失活。
J Biol Chem. 2001 Jul 13;276(28):26291-300. doi: 10.1074/jbc.M011124200. Epub 2001 Apr 23.
9
Novel induction of alpha-lactalbumin-mediated lacdiNAc-R expression in vivo.体内新型诱导α-乳白蛋白介导的乳糖二胺聚糖-R表达
Biochem J. 2000 May 15;348 Pt 1(Pt 1):229-34.
10
Mice infected with Schistosoma mansoni generate antibodies to LacdiNAc (GalNAc beta 1-->4GlcNAc) determinants.感染曼氏血吸虫的小鼠会产生针对LacdiNAc（β-1,4连接的N-乙酰半乳糖胺和N-乙酰葡糖胺）决定簇的抗体。
Glycobiology. 1999 Oct;9(10):1029-35. doi: 10.1093/glycob/9.10.1029.