Urish Kenneth L, Vella Joseph B, Okada Masaho, Deasy Bridget M, Tobita Kimimasa, Keller Bradley B, Cao Baohong, Piganelli Jon D, Huard Johnny
Department of Orthopaedics and Rehabilitation, and Department of Surgery, Penn State Milton S. Hershey Medical Center, Hershey, PA 17033, USA.
Mol Biol Cell. 2009 Jan;20(1):509-20. doi: 10.1091/mbc.e08-03-0274. Epub 2008 Nov 12.
Stem cells are classically defined by their multipotent, long-term proliferation, and self-renewal capabilities. Here, we show that increased antioxidant capacity represents an additional functional characteristic of muscle-derived stem cells (MDSCs). Seeking to understand the superior regenerative capacity of MDSCs compared with myoblasts in cardiac and skeletal muscle transplantation, our group hypothesized that survival of the oxidative and inflammatory stress inherent to transplantation may play an important role. Evidence of increased enzymatic and nonenzymatic antioxidant capacity of MDSCs were observed in terms of higher levels of superoxide dismutase and glutathione, which appears to confer a differentiation and survival advantage. Further when glutathione levels of the MDSCs are lowered to that of myoblasts, the transplantation advantage of MDSCs over myoblasts is lost when transplanted into both skeletal and cardiac muscles. These findings elucidate an important cause for the superior regenerative capacity of MDSCs, and provide functional evidence for the emerging role of antioxidant capacity as a critical property for MDSC survival post-transplantation.
干细胞的经典定义是其具有多能性、长期增殖能力和自我更新能力。在此，我们表明抗氧化能力增强是肌肉来源干细胞（MDSCs）的另一个功能特征。为了理解与成肌细胞相比，MDSCs在心脏和骨骼肌移植中具有更强再生能力的原因，我们团队推测移植过程中固有的氧化应激和炎症应激的存活可能起着重要作用。从超氧化物歧化酶和谷胱甘肽水平较高来看，观察到MDSCs的酶促和非酶促抗氧化能力增强的证据，这似乎赋予了分化和存活优势。进一步地，当MDSCs的谷胱甘肽水平降低到成肌细胞的水平时，将其移植到骨骼肌和心脏肌肉中，MDSCs相对于成肌细胞的移植优势就会丧失。这些发现阐明了MDSCs具有更强再生能力的一个重要原因，并为抗氧化能力作为MDSCs移植后存活的关键特性这一新兴作用提供了功能证据。
