Yang Jiangke, Zhang Bo, Yan Yunjun
Key Laboratory of Molecular Bio-physics, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China.
Appl Biochem Biotechnol. 2009 Nov;159(2):355-65. doi: 10.1007/s12010-008-8419-5. Epub 2008 Nov 13.
Pseudomonas lipases are important biocatalysts widely used in a variety of industrial fields. An extracellular lipase gene lipA with 1,854-bp open reading frame was cloned from Pseudomonas fluorescens 26-2. The multialignment assay of the putative amino acid and the secondary structure prediction revealed this enzyme could be classified into the lipolytic subfamily I.3 and secreted via adenosine-triphosphate-binding cassette pathway. The lipA gene was integrated into Pichia pastoris GS115, and the methanol-inducible recombinants with Mut(S) and Mut(+) phenotypes were acquired. The characteristics and the transesterification capacity shown by this enzyme suggested it is a useful biocatalyst for biodiesel preparation.
假单胞菌脂肪酶是重要的生物催化剂，广泛应用于各种工业领域。从荧光假单胞菌26-2中克隆出一个具有1854个碱基对开放阅读框的细胞外脂肪酶基因lipA。对推测的氨基酸进行多序列比对分析和二级结构预测表明，该酶可归类为脂解亚家族I.3，并通过三磷酸腺苷结合盒途径分泌。将lipA基因整合到毕赤酵母GS115中，获得了具有Mut(S)和Mut(+)表型的甲醇诱导重组体。该酶所表现出的特性和酯交换能力表明它是生物柴油制备中一种有用的生物催化剂。
