贝类作为生物标志物的适宜性：通过 Aroclor 1254 诱导淡水贝类中乙氧基、戊氧基和苯氧基 resorufin-O-脱烷基酶的产生。

The suitability of gastropods as biomarkers: Induction of ethoxy-, pentoxy- and benzoxyresorufin-O-dealkylase in limnetic gastropods via aroclor 1254.

机构信息

Institute of Ecological Chemistry, GSF, D-85758, Neuherberg, Germany.

出版信息

Environ Sci Pollut Res Int. 1997;4(4):183-8. doi: 10.1007/BF02986340.

PMID:19005796

Abstract

The measurement and induction of mixed function oxyigenases (MFOs) of limnetic gastropods were investigated to estimate their suitability as biomarkers. A determination of MFO activities was performed through the measurement of ethoxyresorufin-O-dealkylase (EROD), pentoxy-(PROD) and benzoxyresorufin-O-dealkylase-activity (BROD). Optimal measuring conditions of these activities were investigated in preparations of the digestive gland of 4 species of limnetic gastropods. Results indicate that MFO-activity inhibiting substances are accumulating in the microsomal pellet, the fraction commonly used for the measurement of MFO-activities. Therefore, the fraction used for induction studies was the postmitochondrial supernatant (PMS). EROD, PROD, and BROD activity of Planorbis planorbis and Planorbis carinatus were measured after a treatment with Aroclor 1254 for 1-17 days. Maximal induction of EROD and PROD were 6 and 10 times the value in the control group of P. carinatus, respectively induction in P. planorbis was lower. BROD-activity could not been measured in P. planorbis. In P. carinatus, BROD-activity increased to a maximum of 2 times after treatment with Aroclor 1254. The level of induction of EROD-activity is comparable to results described in analog studies with fish.

摘要

研究了淡水腹足类动物混合功能氧化酶（MFO）的测量和诱导，以评估其作为生物标志物的适用性。通过测量乙氧基Resorufin-O-脱烷基酶（EROD）、戊氧基-（PROD）和苯氧基Resorufin-O-脱烷基酶活性（BROD）来确定 MFO 活性。在 4 种淡水腹足类动物的消化腺制备物中研究了这些活性的最佳测量条件。结果表明，MFO 活性抑制物质在微粒体沉淀中积累，该沉淀通常用于测量 MFO 活性。因此，用于诱导研究的部分是线粒体后上清液（PMS）。用 Aroclor 1254 处理 1-17 天后，测量了 P. planorbis 和 P. carinatus 的 EROD、PROD 和 BROD 活性。P. carinatus 的 EROD 和 PROD 的最大诱导分别是对照组的 6 倍和 10 倍，而 P. planorbis 的诱导较低。在 P. planorbis 中，无法测量 BROD 活性。在 P. carinatus 中，用 Aroclor 1254 处理后，BROD 活性增加到最大 2 倍。EROD 活性的诱导水平与鱼类类似研究中描述的结果相当。