Ezaki I, Kanda H, Sakai K, Fukui N, Shingu M, Nobunaga M, Watanabe T
Department of Clinical Immunology, Kyushu University, Beppu, Japan.
Arthritis Rheum. 1991 Mar;34(3):343-50. doi: 10.1002/art.1780340312.
The complete nucleotide sequences of the variable region genes of the heavy and light polypeptide chains of a human monoclonal rheumatoid factor (RF) produced from a human-mouse heterohybridoma were determined. The antibody, designated YES8c, contained V kappa III, J kappa 2, VH1, JH4, and a D gene segment of 9 amino acids. The nucleotide sequences and the deduced amino acid sequences of the light chain variable region were remarkably homologous (97-98%) to previously described RF of the Wa idiotypic family (PAY, GLO, CUR, FLO, and GAR) and to that of a V kappa III germline gene (Humkv325). The YES8c heavy chain variable region gene was most closely related to the VH1 gene of the restricted human fetal repertoire, designated 51p1, and also to 3 rearranged VH1 genes that were recently isolated from patients with chronic lymphocytic leukemia. These results suggest that variable region genes of RFs are highly conserved and that YES8c VH, as well as V kappa, may be identical to heavy and light chains expressed during early B cell development.
测定了由人-鼠异源杂交瘤产生的人单克隆类风湿因子（RF）重链和轻链可变区基因的完整核苷酸序列。该抗体命名为YES8c，包含VκIII、Jκ2、VH1、JH4以及一个由9个氨基酸组成的D基因片段。轻链可变区的核苷酸序列和推导的氨基酸序列与先前描述的Wa独特型家族的RF（PAY、GLO、CUR、FLO和GAR）以及VκIII种系基因（Humkv325）具有显著的同源性（97-98%）。YES8c重链可变区基因与限制性人类胎儿库的VH1基因（命名为51p1）以及最近从慢性淋巴细胞白血病患者中分离出的3个重排VH1基因关系最为密切。这些结果表明，RF的可变区基因高度保守，并且YES8c的VH以及Vκ可能与早期B细胞发育过程中表达的重链和轻链相同。
