Min Fang, Si Jin-ping, Huang Wen-hua, Huang Hua-hong, Lou Shao-qing, Zhu Guan-quan
Zhejiang Normal University, Jinhua 321000, China.
Zhongguo Zhong Yao Za Zhi. 2008 Aug;33(15):1849-53.
To study the content variation of furmarid acid and isofraxidin in Sarcandra glabra from 21 different provenances and provide the basis for resource utilization and quality optimization of S. glabra.
HPLC method was developed to determine the contents of furmarid acid and isofraxidin in 330 samples of S. glabra which were collected respectively from 21 different provenances.
There were significant differences in the contents of isofraxidin and furmarid acid in S. glabra from different provenances. The contents of isofraxidin and furmarid acid dropped off from low altitude to high altitude, which were also close with longitude and latitude. The content of isofraxidin in S. glabra at central area of natural distribution was the highest. The different parts of the plant had different results, the influence on the contents of the acitive components in stem were more obvious than the leaf.
This simple, accurate and reproducible method could be use to determine the contents of furmarid acid and isofraxidin in S. glabra. The results represented the status of medicines quality and difference of Chinese S. glabra. These agreed with the traditional views that the medicines quality of Sarcandra glabra in Jiangxi, Fujian, Zhejiang was better. These provenances were considered as important areas of medicines breeding and bases building on S. glabra in future.
研究21个不同产地九节龙中岩白菜素和异嗪皮啶的含量变化，为九节龙资源利用和质量优化提供依据。
采用高效液相色谱法测定分别来自21个不同产地的330份九节龙样品中岩白菜素和异嗪皮啶的含量。
不同产地九节龙中异嗪皮啶和岩白菜素的含量存在显著差异。异嗪皮啶和岩白菜素的含量从低海拔到高海拔逐渐降低，且与经度和纬度也有一定关系。九节龙自然分布中心区域的异嗪皮啶含量最高。植物的不同部位结果不同，茎对活性成分含量的影响比叶更明显。
该方法简便、准确、重现性好，可用于测定九节龙中岩白菜素和异嗪皮啶的含量。结果反映了九节龙药材质量状况及差异，与传统认为江西、福建、浙江等地九节龙药材质量较好的观点相符。这些产地可作为今后九节龙药材选育和基地建设的重要区域。
