Billiot Eugene, Billiot Fereshteh, Warner Isiah M
Department of Physical and Life Sciences, Texas A&M Corpus Christi, Corpus Christi, TX 78412, USA.
J Chromatogr Sci. 2008 Oct;46(9):757-63. doi: 10.1093/chromsci/46.9.757.
This manuscript discusses the results of studies that were performed to determine optimum capillary electrophoresis (CE) conditions for the enantiomeric resolution of twelve chiral analytes with eight amino acid based polymeric surfactants. The parameters that were optimized include pH, buffer type, and concentration of surfactant. The results indicated that the optimum conditions for enantiomeric separations with the amino acid based polymeric surfactants examined in this study using CE were analyte dependent, not surfactant dependent. In other words, the optimum conditions for a particular analyte were the same for all the amino acid based polymeric surfactants examined in this study. The results of these studies indicate that when using a large group of related amino acid based polymeric surfactants only a few surfactants need to be optimized for each analyte under study. These studies were limited to anionic surfactants that contain the amino acids glycine, L-alanine, L-valine, and L-leucine only. No inference can be necessarily drawn about surfactants containing other types of amino acids such as threonine and serine, which contain extra heteroatoms, or phenylalanine that has an aromatic moiety.
本手稿讨论了为确定使用八种基于氨基酸的聚合表面活性剂对十二种手性分析物进行对映体拆分的最佳毛细管电泳（CE）条件而开展的研究结果。优化的参数包括pH值、缓冲液类型和表面活性剂浓度。结果表明，本研究中使用CE对基于氨基酸的聚合表面活性剂进行对映体分离的最佳条件取决于分析物，而非表面活性剂。换句话说，对于特定分析物，本研究中所检测的所有基于氨基酸的聚合表面活性剂的最佳条件都是相同的。这些研究结果表明，当使用一大组相关的基于氨基酸的聚合表面活性剂时，对于每种所研究的分析物，仅需优化少数几种表面活性剂。这些研究仅限于仅含有甘氨酸、L-丙氨酸、L-缬氨酸和L-亮氨酸的阴离子表面活性剂。对于含有其他类型氨基酸（如含有额外杂原子的苏氨酸和丝氨酸或具有芳香部分的苯丙氨酸）的表面活性剂，不一定能得出推断。
