Greenberg Joshua I, Suliman Ahmed, Barillas Samuel, Angle Niren
Section of Vascular and Endovascular Surgery, Department of Surgery, School of Medicine, University of California San Diego, San Diego, California, USA.
Methods Enzymol. 2008;444:159-74. doi: 10.1016/S0076-6879(08)02807-3.
Ischemia and ischemia-reperfusion (I/R) events are distinct but interrelated processes etiologic to the most prevalent human diseases. A delicate balance exists whereby ischemic injury can result in beneficial angiogenesis or in detrimental reperfusion injury overwhelming the organism. Here, we describe in vivo models of ischemia and ischemia-reperfusion injury with emphasis on murine hindlimb ischemia models. We also provide a brief introduction to murine myocardial ischemia experiments. Each model is described in the context of human disease. Emphasis is made on the strengths and weaknesses of the available techniques, particularly as it relates to data analysis, interpretation, and translational relevance.
缺血和缺血再灌注（I/R）事件是不同但相互关联的过程，是人类最常见疾病的病因。存在一种微妙的平衡，即缺血性损伤可导致有益的血管生成，也可导致有害的再灌注损伤，从而使机体不堪重负。在此，我们描述了缺血和缺血再灌注损伤的体内模型，重点是小鼠后肢缺血模型。我们还简要介绍了小鼠心肌缺血实验。每个模型都在人类疾病的背景下进行了描述。重点介绍了现有技术的优缺点，特别是与数据分析、解释及转化相关性有关的方面。
