Zhu X M, Han T, Sargent I L, Wang Y L, Yao Y Q
Department of Obstetrics and Gynecology, Tangdu Hospital, The Fourth Military Medical University, Xinsi Road, Xi'an 710038, China.
Placenta. 2009 Jan;30(1):74-8. doi: 10.1016/j.placenta.2008.09.013. Epub 2008 Nov 13.
The regulation of trophoblast cell invasion is a crucial aspect of implantation and placental development. Evidence indicates that the uterine microenvironment exerts important influence over trophoblast cell invasion. However, the precise effect of decidual cells on trophoblast cell invasion remained unidentified. In the present study, a cell line representative of normal human trophoblast (B6Tert) was used to examine the effect of decidual stromal cell conditioned media (DSCM) on trophoblast cell invasion. In vitro assay showed the concentration-dependent effect of DSCM on B6Tert cell invasion. RT-PCR and gelatin zymography demonstrated that DSCM evidently produced an effect on the mRNA expression and proenzyme production of MMP-2 in a dose-dependent manner, but exerted no effect on mRNA expression and proenzyme production of MMP-9. The data indicates that the decidual microenvironment may exert the key control for trophoblast cell invasion mainly through influencing MMP-2 expression.
滋养层细胞侵袭的调控是着床和胎盘发育的关键环节。有证据表明，子宫微环境对滋养层细胞侵袭具有重要影响。然而，蜕膜细胞对滋养层细胞侵袭的确切作用仍不明确。在本研究中，使用一种代表正常人滋养层的细胞系（B6Tert）来检测蜕膜基质细胞条件培养基（DSCM）对滋养层细胞侵袭的影响。体外试验显示DSCM对B6Tert细胞侵袭具有浓度依赖性效应。逆转录聚合酶链反应（RT-PCR）和明胶酶谱分析表明，DSCM对基质金属蛋白酶-2（MMP-2）的mRNA表达和酶原产生有明显的剂量依赖性影响，但对MMP-9的mRNA表达和酶原产生没有影响。数据表明，蜕膜微环境可能主要通过影响MMP-2的表达对滋养层细胞侵袭发挥关键调控作用。
