Engel Elisabeth, Martínez Elena, Mills Chris A, Funes Miriam, Planell Josep A, Samitier Josep
Bio/Non-Bio Interactions for Regenerative Medicine Group, Institute for Bioengineering of Catalonia (IBEC), Josep Samitier 1-5, 08028 Barcelona, Spain.
Ann Anat. 2009 Jan;191(1):136-44. doi: 10.1016/j.aanat.2008.07.013. Epub 2008 Sep 27.
Recent studies on 2D substrates have revealed the importance of surface properties in affecting cell behaviour. In particular, surface topography appears to influence and direct cell migration. The development of new technologies of hot embossing and micro-imprinting has made it possible to study cell interactions with controlled micro features and to determine how these features can affect cell behaviour. Several studies have been carried out on the effect of microstructures on cell adhesion, cell guidance and cell proliferation. However, there is still a lack of knowledge on how these features affect mesenchymal stem cell differentiation. This study was designed to evaluate whether highly controlled microstructures on PMMA could induce rMSC differentiation into an osteogenic lineage. Structured PMMA was seeded with rMSC and cell number; cell morphology and cell differentiation were evaluated. Results confirm that microstructures not only affect cell proliferation and alignment but also have a synergistic effect with osteogenic medium on rMSC differentiation into mature osteoblasts.
近期关于二维基质的研究揭示了表面性质在影响细胞行为方面的重要性。特别是，表面形貌似乎会影响并引导细胞迁移。热压印和微压印等新技术的发展使得研究细胞与可控微观特征的相互作用以及确定这些特征如何影响细胞行为成为可能。已经开展了多项关于微观结构对细胞黏附、细胞引导和细胞增殖影响的研究。然而，对于这些特征如何影响间充质干细胞分化仍缺乏了解。本研究旨在评估聚甲基丙烯酸甲酯（PMMA）上高度可控的微观结构是否能诱导大鼠间充质干细胞（rMSC）分化为成骨谱系。将rMSC接种到结构化的PMMA上，并对细胞数量、细胞形态和细胞分化进行评估。结果证实，微观结构不仅影响细胞增殖和排列，而且在rMSC分化为成熟成骨细胞的过程中与成骨培养基具有协同作用。
