Kato K, Agatsuma T, Tanabe T, Masuko T, Hashimoto Y
Department of Hygienic Chemistry, Pharmaceutical Institute, Tohoku University, Sendai.
Jpn J Cancer Res. 1991 Feb;82(2):206-12. doi: 10.1111/j.1349-7006.1991.tb01830.x.
Release of granule enzyme(s) (BLT esterase) in the antibody dependent lymphokine-activated killer (LAK) cell-mediated cytotoxic reaction (LAK ADCC) was studied using LAK cells induced from murine splenocytes and thymocytes, various human tumor cells and relevant monoclonal antibodies (mAbs) to the tumor cells. BLT esterase was not significantly released from LAK cells in direct LAK cell-mediated cytotoxic reactions (LAK CMC). However, cultures of LAK cells and IgG-coated target tumor cells resulted in release of the enzyme concomitantly with target cell lysis, although esterase release proceeded faster than target cell lysis. Anti-LFA-1 mAb showed an inhibitory effect on LAK CMC but not on either LAK ADCC or BLT esterase release in the ADCC. These results indicate that exocytosis of granule enzyme from LAK cells is triggered by stimulation of Fc receptor on LAK cells and that LAK CMC and LAK ADCC differ in their lytic mechanism in terms of the release of BLT esterase.
利用从小鼠脾细胞和胸腺细胞诱导产生的淋巴因子激活的杀伤（LAK）细胞、各种人类肿瘤细胞以及针对肿瘤细胞的相关单克隆抗体（mAb），研究了在抗体依赖性淋巴因子激活的杀伤（LAK）细胞介导的细胞毒性反应（LAK ADCC）中颗粒酶（BLT酯酶）的释放情况。在直接的LAK细胞介导的细胞毒性反应（LAK CMC）中，LAK细胞未显著释放BLT酯酶。然而，LAK细胞与IgG包被的靶肿瘤细胞共培养时，酶的释放与靶细胞裂解同时发生，尽管酯酶释放比靶细胞裂解更快。抗LFA-1 mAb对LAK CMC有抑制作用，但对LAK ADCC或ADCC中BLT酯酶的释放均无抑制作用。这些结果表明，LAK细胞颗粒酶的胞吐作用是由LAK细胞上Fc受体的刺激触发的，并且就BLT酯酶的释放而言，LAK CMC和LAK ADCC的裂解机制不同。
