Pontes Ana Rosa Botelho, Almeida Maria das Graças Carvalho, Xavier Marília Brasil, Quaresma Juarez Antonio Simões, Yassui Edna Aoba
Universidade Federal do Pará, Belém, PA.
Rev Bras Enferm. 2008;61 Spec No:734-7. doi: 10.1590/s0034-71672008000700013.
Studies have demonstrated high sensibility of the polimerase chain reaction (PCR) technique in the identification of the Mycobacterium leprae DNA . This study aimed to evalue the PCR sensibility at the detection of the M. leprae DNA in nasal swab of leprosy patients and to compare the results with the bacilloscopy and multibacillary (MBs) and paucibacilares (PBs) forms. Nasal secretion samples of 24 leprosy patients were collected, and were preserved in one and two lise's solution. The PCR results were highly significant (p <0.0000) and they revealed grater sensibility than bacilloscopy, in several clinical forms. Nevertheless, still different studies are necessary, testing new markers and preservatives, with the purpose of lifting up the sensibility of this technique, in nasal secretion samples.
研究表明，聚合酶链反应（PCR）技术在鉴定麻风分枝杆菌DNA方面具有很高的灵敏度。本研究旨在评估PCR检测麻风患者鼻拭子中麻风分枝杆菌DNA的灵敏度，并将结果与细菌学检查以及多菌型（MBs）和少菌型（PBs）进行比较。收集了24例麻风患者的鼻分泌物样本，并保存在1号和2号利氏溶液中。PCR结果具有高度显著性（p<0.0000），在几种临床类型中，其显示出比细菌学检查更高的灵敏度。然而，仍需要进行不同的研究，测试新的标志物和防腐剂，以提高该技术在鼻分泌物样本中的灵敏度。
