Dreux Marlène, Cosset François-Loïc
Universit de Lyon, (UCB-Lyon1), Lyon, IFR128, France.
Methods Mol Biol. 2009;510:427-38. doi: 10.1007/978-1-59745-394-3_32.
Infectious HCV pseudoparticles (HCVpp) can be assembled by display of unmodified and functional HCV glycoproteins on retroviral and lentiviral core particles. HCVpp have been shown to mimic the early infection steps of parental HCV. The presence of a marker gene packaged within these HCV pseudoparticles allows reliable and fast determination of infectivity mediated by the HCV glycoproteins. With this highly flexible system, E1E2 from a broad range of HCV strains can be investigated, including autologous HCV strains from patients' virus, and it has allowed careful investigation of the humoral response to HCV.
传染性丙型肝炎病毒假颗粒（HCVpp）可通过在逆转录病毒和慢病毒核心颗粒上展示未修饰的功能性丙型肝炎病毒糖蛋白来组装。HCVpp已被证明可模拟亲本丙型肝炎病毒的早期感染步骤。这些HCV假颗粒中包装的标记基因的存在使得能够可靠且快速地确定由丙型肝炎病毒糖蛋白介导的感染性。利用这个高度灵活的系统，可以研究来自广泛丙型肝炎病毒株的E1E2，包括来自患者病毒的同源丙型肝炎病毒株，并且它使得能够仔细研究针对丙型肝炎病毒的体液反应。
