Emre M H, Polat A, Eşrefoğlu M, Karabulut A B, Gül M
Department of Physiology, Inonu University, Medical School, 44280 Malatya, Turkey.
Acta Physiol Hung. 2008 Dec;95(4):349-63. doi: 10.1556/APhysiol.2008.0001.
The aim of this study was to assess the effect of melatonin and acetylsalicylic acid (ASA) on hepatic damage induced by bile duct ligation (BDL).
Male Sprague-Dawley rats were subjected to either sham operation or common BDL before treatment with ASA, melatonin or vehicle. Hepatic superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) enzyme activities and reduced glutathione (GSH), malondialdehyde (MDA) and nitric oxide (NO) levels were evaluated.
Our results have indicated that BDL caused a significant increase in lipid peroxidation whereas a statistically insignificant decrease in GSH level and some of the antioxidant enzyme activities. Both MEL and ASA administrations, either separately or together, decreased MDA whereas co-administration of MEL with ASA increased GSH levels in BDL rats.
CAT activity and MEL level decreased in the liver tissues of rats with BDL after administration of either melatonin alone or with ASA. However, melatonin and ASA administration increases liver tissue GSH levels in BDL ligated rats
本研究旨在评估褪黑素和乙酰水杨酸（ASA）对胆管结扎（BDL）诱导的肝损伤的影响。
雄性Sprague-Dawley大鼠在接受ASA、褪黑素或赋形剂治疗前，分别接受假手术或普通BDL。评估肝脏超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）的酶活性以及还原型谷胱甘肽（GSH）、丙二醛（MDA）和一氧化氮（NO）的水平。
我们的结果表明，BDL导致脂质过氧化显著增加，而GSH水平和一些抗氧化酶活性在统计学上有不显著的降低。单独或联合给予褪黑素（MEL）和ASA均可降低MDA，而MEL与ASA联合给药可提高BDL大鼠的GSH水平。
单独给予褪黑素或与ASA联合给药后，BDL大鼠肝组织中的CAT活性和MEL水平降低。然而，给予褪黑素和ASA可提高BDL结扎大鼠的肝组织GSH水平
