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2
In vitro protein-primed initiation of pneumococcal phage Cp-1 DNA replication occurs at the third 3' nucleotide of the linear template: a stepwise sliding-back mechanism.肺炎球菌噬菌体Cp-1 DNA复制的体外蛋白质引发起始发生在线性模板的第三个3'核苷酸处：一种逐步回滑机制。
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Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication.噬菌体phi29 DNA聚合酶和末端蛋白亚结构域在蛋白质引发的DNA复制起始过程中赋予特异性的作用。
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Initiation of phi 29 DNA replication occurs at the second 3' nucleotide of the linear template: a sliding-back mechanism for protein-primed DNA replication.φ29 DNA复制的起始发生在线性模板的第二个3'核苷酸处：蛋白质引发DNA复制的滑回机制。
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本文引用的文献

1
Involvement of phage phi29 DNA polymerase and terminal protein subdomains in conferring specificity during initiation of protein-primed DNA replication.噬菌体phi29 DNA聚合酶和末端蛋白亚结构域在蛋白质引发的DNA复制起始过程中赋予特异性的作用。
Nucleic Acids Res. 2007;35(21):7061-73. doi: 10.1093/nar/gkm749. Epub 2007 Oct 2.
2
Structures of phi29 DNA polymerase complexed with substrate: the mechanism of translocation in B-family polymerases.与底物复合的phi29 DNA聚合酶结构：B族聚合酶中的易位机制
EMBO J. 2007 Jul 25;26(14):3494-505. doi: 10.1038/sj.emboj.7601780. Epub 2007 Jul 5.
3
Functional characterization of highly processive protein-primed DNA polymerases from phages Nf and GA-1, endowed with a potent strand displacement capacity.来自噬菌体Nf和GA-1的具有高效链置换能力的高度持续合成的蛋白质引发的DNA聚合酶的功能特性。
Nucleic Acids Res. 2006;34(20):6051-63. doi: 10.1093/nar/gkl769. Epub 2006 Oct 28.
4
His1 and His2 are distantly related, spindle-shaped haloviruses belonging to the novel virus group, Salterprovirus.His1和His2是远缘相关的纺锤形嗜盐病毒，属于新型病毒组盐杆菌病毒属。
Virology. 2006 Jun 20;350(1):228-39. doi: 10.1016/j.virol.2006.02.005. Epub 2006 Mar 10.
5
The phi29 DNA polymerase:protein-primer structure suggests a model for the initiation to elongation transition.phi29 DNA聚合酶：蛋白质引物结构为起始到延伸转变提出了一个模型。
EMBO J. 2006 Mar 22;25(6):1335-43. doi: 10.1038/sj.emboj.7601027. Epub 2006 Mar 2.
6
Constituents of SH1, a novel lipid-containing virus infecting the halophilic euryarchaeon Haloarcula hispanica.SH1的组成成分，一种感染嗜盐广古菌西班牙盐盒菌的新型含脂病毒。
J Virol. 2005 Jul;79(14):9097-107. doi: 10.1128/JVI.79.14.9097-9107.2005.
7
A "slide-back" mechanism for the initiation of protein-primed RNA synthesis by the RNA polymerase of poliovirus.脊髓灰质炎病毒RNA聚合酶引发蛋白质引发的RNA合成的“回滑”机制。
J Biol Chem. 2003 Nov 7;278(45):43951-60. doi: 10.1074/jbc.M307441200. Epub 2003 Aug 22.
8
Functional dissection of a poliovirus cis-acting replication element [PV-cre(2C)]: analysis of single- and dual-cre viral genomes and proteins that bind specifically to PV-cre RNA.脊髓灰质炎病毒顺式作用复制元件[PV-cre(2C)]的功能剖析：对单cre和双cre病毒基因组以及与PV-cre RNA特异性结合的蛋白质的分析
J Virol. 2003 May;77(9):5152-66. doi: 10.1128/jvi.77.9.5152-5166.2003.
9
Biochemical and genetic studies of the VPg uridylylation reaction catalyzed by the RNA polymerase of poliovirus.脊髓灰质炎病毒RNA聚合酶催化的VPg尿苷酸化反应的生化及遗传学研究
J Virol. 2003 Jan;77(2):891-904. doi: 10.1128/jvi.77.2.891-904.2003.
10
Sequence requirements for protein-primed initiation and elongation of phage O29 DNA replication.噬菌体O29 DNA复制的蛋白质引发起始和延伸的序列要求。
J Biol Chem. 2000 Dec 22;275(51):40547-53. doi: 10.1074/jbc.M007170200.

噬菌体φ29和Nf末端蛋白引发结构域指定内部模板核苷酸以启动DNA复制。

Phage phi29 and Nf terminal protein-priming domain specifies the internal template nucleotide to initiate DNA replication.

作者信息

Longás Elisa, Villar Laurentino, Lázaro José M, de Vega Miguel, Salas Margarita

机构信息

Instituto de Biología Molecular "Eladio Viñuela," Centro de Biología Molecular "Severo Ochoa," Consejo Superior de Investigaciones Científicas-Universidad Autónoma C/Nicolás Cabrera 1, Universidad Autónoma, Cantoblanco, 28049 Madrid, Spain.

出版信息

Proc Natl Acad Sci U S A. 2008 Nov 25;105(47):18290-5. doi: 10.1073/pnas.0809882105. Epub 2008 Nov 14.

DOI:10.1073/pnas.0809882105
PMID:19011105
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2587616/
Abstract

Bacteriophages phi29 and Nf from Bacillus subtilis start replication of their linear genome at both DNA ends by a protein-primed mechanism, by which the DNA polymerase, in a template-instructed reaction, adds 5'-dAMP to a molecule of terminal protein (TP) to form the initiation product TP-dAMP. Mutational analysis of the 3 terminal thymines of the Nf DNA end indicated that initiation of Nf DNA replication is directed by the third thymine on the template, the recovery of the 2 terminal nucleotides mainly occurring by a stepwise sliding-back mechanism. By using chimerical TPs, constructed by swapping the priming domain of the related phi29 and Nf proteins, we show that this domain is the main structural determinant that dictates the internal 3' nucleotide used as template during initiation.

摘要

来自枯草芽孢杆菌的噬菌体phi29和Nf通过蛋白质引发机制在其线性基因组的两端起始复制，在该机制中，DNA聚合酶在模板指导的反应中，将5'-dAMP添加到末端蛋白（TP）分子上以形成起始产物TP-dAMP。对Nf DNA末端的3个末端胸腺嘧啶进行的突变分析表明，Nf DNA复制的起始由模板上的第三个胸腺嘧啶指导，2个末端核苷酸的恢复主要通过逐步回滑机制发生。通过使用通过交换相关phi29和Nf蛋白的引发结构域构建的嵌合TP，我们表明该结构域是决定起始过程中用作模板的内部3'核苷酸的主要结构决定因素。