Bauer P I, Kline K, Kun E
Laboratory for Environmental Toxicology and Chemistry, Romberg Tiburon Center, San Francisco State University, Tiburon, California 94920.
Proc Soc Exp Biol Med. 1991 Apr;196(4):396-400. doi: 10.3181/00379727-196-43205.
The poly(ADP-ribose) polymerase or transferase (ADPRT) activity of developing embryos of Mytilus edulis increases with the progression of larval growth. ADPRT protein was partially purified from 2-hr-old embryos and identified by gel electrophoresis and immunotransblot, demonstrating cross-reactivity with anti-ADPRT IgG produced against the calf thymus enzyme. Two inhibitors of ADPRT, benzamide, competing with NAD at the nicotinamide binding site, and 6-amino-1,2-benzopyrone, which competes with DNA at the DNA binding site(s), both selectively arrest differentiation at the prodissoconch stage. The DNA site-oriented inhibitor, 6-amino-1,2-benzopyrone, has a much larger differentiation arresting effect than benzamide. The arrest of differentiation by 6-amino-1,2-benzopyrone is reversible. A probable ecotoxicity of ADPRT ligands on mussel differentiation is proposed.
紫贻贝发育中胚胎的聚（ADP - 核糖）聚合酶或转移酶（ADPRT）活性随着幼虫生长的进程而增加。ADPRT蛋白从2小时龄的胚胎中部分纯化，并通过凝胶电泳和免疫印迹进行鉴定，显示出与针对小牛胸腺酶产生的抗ADPRT IgG具有交叉反应性。两种ADPRT抑制剂，在烟酰胺结合位点与NAD竞争的苯甲酰胺，以及在DNA结合位点与DNA竞争的6 - 氨基 - 1,2 - 苯并吡喃，都在原壳幼虫阶段选择性地阻止分化。面向DNA位点的抑制剂6 - 氨基 - 1,2 - 苯并吡喃比苯甲酰胺具有更大的阻止分化的作用。6 - 氨基 - 1,2 - 苯并吡喃引起的分化停滞是可逆的。提出了ADPRT配体对贻贝分化可能的生态毒性。
