Kaushik A, Reininger L, Kelsoe G, Jaton J C, Bona C
Department of Microbiology, Mount Sinai School of Medicine, New York.
Eur J Immunol. 1991 Mar;21(3):827-30. doi: 10.1002/eji.1830210344.
The contribution of VH11 gene family to the development of the primary B cell repertoire has been studied by analyzing 1.8 x 10(4) mitogen induced B lymphocyte colonies. The data demonstrate that VH11 family is predominantly expressed among neonatal splenic as well as adult peritoneal B cell colonies, both rich in Ly-1+ B cells. VH11 gene family expression among B splenocytes decreases during ontogeny and VH11 family pairs stochastically with different V kappa families among mitogen-activated neonatal B cell colonies, which are representative of an antigen unselected B cell repertoire. Thus, an increased VH11 expression among peritoneal and neonatal B cells points towards its biased expression among Ly-1+ B lymphocytes. The restricted V gene rearrangements and VH11-V kappa 9 pairing observed among anti-bromelain-treated mouse red blood cells autoantibodies are likely to be an outcome of both intrinsic gene recombination processes per se as well as selection by an autoantigen and/or local selective environmental factors.
通过分析1.8×10⁴个有丝分裂原诱导的B淋巴细胞集落，研究了VH11基因家族对初级B细胞库发育的贡献。数据表明，VH11家族在富含Ly-1⁺ B细胞的新生脾脏以及成年腹膜B细胞集落中占主导表达。B脾细胞中VH11基因家族的表达在个体发育过程中降低，并且在有丝分裂原激活的新生B细胞集落中，VH11家族与不同的Vκ家族随机配对，这些集落代表未被抗原选择的B细胞库。因此，腹膜和新生B细胞中VH11表达的增加表明其在Ly-1⁺ B淋巴细胞中存在偏向性表达。在抗菠萝蛋白酶处理的小鼠红细胞自身抗体中观察到的受限V基因重排和VH11-Vκ9配对，可能是内在基因重组过程本身以及自身抗原和/或局部选择环境因素选择的结果。
