Malley R C, Muller H K, Norval M, Woods G M
Menzies Research Institute, Clinical School, University of Tasmania, Private Bag 29, Hobart, Tasmania 7000, Australia.
Cell Immunol. 2009;255(1-2):33-40. doi: 10.1016/j.cellimm.2008.09.004. Epub 2008 Nov 13.
To ascertain the influence of vitamin D3 and its metabolites on the function of the skin immune system and the induction of the contact hypersensitivity (CHS) response, a population of vitamin D3-deficient BALB/c mice was established, through dietary vitamin D3 restriction and limitation of exposure to UVB irradiation. Vitamin D3 normal female mice had higher CHS responses than their male counterparts, and dietary vitamin D3 deficiency significantly increased the CHS responses in male, but not in female, mice. This change in the vitamin D3-deficient male mice was not due to an alteration in skin dendritic cell function including antigen carriage, migration or costimulatory molecule expression. In addition, 18 h after sensitisation, the lymph node populations in the vitamin D3-deficient and normal male mice showed similar proliferation and IFN-gamma production. However, during the sensitisation phase of CHS, there was lower lymphocyte recruitment to the skin draining lymph nodes of the vitamin D3-deficient and normal male mice compared with their female counterparts which could account for the difference between the sexes in the extent of the CHS response. These results indicate the vitamin D system can influence cutaneous immune responses in male mice, but this did not occur through the modulation of the dendritic cell functions analysed.
为确定维生素D3及其代谢产物对皮肤免疫系统功能和接触性超敏反应（CHS）诱导的影响，通过饮食限制维生素D3和限制紫外线B照射，建立了一群维生素D3缺乏的BALB/c小鼠。维生素D3正常的雌性小鼠比雄性小鼠具有更高的CHS反应，饮食中维生素D3缺乏显著增加了雄性小鼠的CHS反应，但对雌性小鼠没有影响。维生素D3缺乏的雄性小鼠的这种变化并非由于皮肤树突状细胞功能的改变，包括抗原携带、迁移或共刺激分子表达。此外，致敏18小时后，维生素D3缺乏和正常的雄性小鼠的淋巴结群体显示出相似的增殖和IFN-γ产生。然而，在CHS致敏阶段，与雌性小鼠相比，维生素D3缺乏和正常的雄性小鼠引流皮肤的淋巴结中淋巴细胞募集较少，这可以解释两性在CHS反应程度上的差异。这些结果表明维生素D系统可以影响雄性小鼠的皮肤免疫反应，但这并非通过所分析的树突状细胞功能的调节而发生。
