Powell Fiona, Lawson Michelle, Rothwell Lisa, Kaiser Pete
Institute for Animal Health, Compton, Berkshire RG20 7NN, UK.
Dev Comp Immunol. 2009 Apr;33(4):540-6. doi: 10.1016/j.dci.2008.10.005. Epub 2008 Nov 12.
The cDNAs of three turkey CD markers, CD4, CD8alpha and CD28, were identified by screening a turkey cDNA library. The coding regions of the chicken and turkey genes are highly conserved, with 91.3-96.1% nucleotide (nt) and 84.2-95.5% amino acid (aa) identity. Identity was less conserved between avian CD markers and their mammalian homologues, ranging from 44.7 to 59.8% and 22.4 to 50.4% at the nt and aa levels, respectively. Anti-chicken CD8alpha and CD28 monoclonal antibodies were demonstrated to specifically cross-react with turkey CD8alpha and CD28, respectively.
通过筛选火鸡cDNA文库，鉴定出三种火鸡CD标志物（CD4、CD8α和CD28）的cDNA。鸡和火鸡基因的编码区高度保守，核苷酸（nt）同一性为91.3 - 96.1%，氨基酸（aa）同一性为84.2 - 95.5%。禽类CD标志物与其哺乳动物同源物之间的同一性保守性较低，在核苷酸和氨基酸水平上分别为44.7%至59.8%和22.4%至50.4%。抗鸡CD8α和CD28单克隆抗体分别被证明能与火鸡CD8α和CD28特异性交叉反应。
