Mitchell Judy A, Brooks Harriet, Shiu Kai-Biu, Brownlie Joe, Erles Kerstin
Department of Pathology and Infectious Diseases, The Royal Veterinary College, Hawkshead Lane, North Mymms, Hatfield, Hertfordshire AL9 7TA, UK.
J Virol Methods. 2009 Feb;155(2):136-42. doi: 10.1016/j.jviromet.2008.10.008. Epub 2008 Nov 28.
Canine respiratory coronavirus (CRCoV) has been detected recently in dogs with canine infectious respiratory disease and is involved in the clinical disease complex. CRCoV is a group 2 coronavirus most closely related to bovine coronavirus and human coronavirus OC43. A real-time PCR assay was developed for the detection of CRCoV. The assay was validated against cell culture grown virus and shown to have a high level of sensitivity. A range of tissue samples were collected from dogs at a re-homing centre with a history of endemic respiratory disease. The samples were tested using a conventional nested PCR assay and CRCoV was quantitated by real-time PCR. CRCoV was detected most frequently in the nasal mucosa, nasal tonsil and trachea. It was also detected in the lung, and bronchial lymph node. Of the enteric tissues, only one mesenteric lymph node sample was positive. In addition two colon samples were positive for CRCoV by nested PCR only. In conclusion, CRCoV appears to infect the upper respiratory tract preferentially. The CRCoV real-time PCR assay has proved to be a highly specific and sensitive assay that can be applied for diagnostic purposes as well as to investigate further the tissue tropism of CRCoV.
犬呼吸道冠状病毒（CRCoV）最近在患有犬传染性呼吸道疾病的犬只中被检测到，并与临床疾病复合体有关。CRCoV是一种2型冠状病毒，与牛冠状病毒和人冠状病毒OC43关系最为密切。开发了一种用于检测CRCoV的实时PCR检测方法。该检测方法针对细胞培养生长的病毒进行了验证，并显示出高度的敏感性。从一个有地方性呼吸道疾病病史的重新安置中心的犬只收集了一系列组织样本。使用传统的巢式PCR检测方法对样本进行检测，并通过实时PCR对CRCoV进行定量。CRCoV在鼻黏膜、鼻扁桃体和气管中最常被检测到。在肺和支气管淋巴结中也检测到了。在肠道组织中，只有一个肠系膜淋巴结样本呈阳性。此外，仅通过巢式PCR检测，两个结肠样本的CRCoV呈阳性。总之，CRCoV似乎优先感染上呼吸道。CRCoV实时PCR检测方法已被证明是一种高度特异性和敏感性的检测方法，可用于诊断目的以及进一步研究CRCoV的组织嗜性。
