Cho Eun Young, Lee Chae Jin, Son Keun Su, Kim Yoo Jung, Kim Sun Jung
Department of Life Science, Dongguk University, Seoul 100-715, Republic of Korea.
Gene. 2009 Jan 15;429(1-2):44-8. doi: 10.1016/j.gene.2008.10.008. Epub 2008 Nov 1.
In this study, we identified and characterized a mouse brain-enriched unigene (msvop) after performing digital differential display. Msvop was the mouse ortholog of Xenopus synaptic vesicle-2-associated protein (svop), the molecular characteristics of which were unknown. The 3125-bp full-length cDNA encoded a 548-aa protein of approximately 60 kDa. A strong promoter element was found in the -200 to -100 bp region in both NG108-15 and HEK293 cells. RT-PCR and in situ hybridization analysis confirmed that msvop was strictly expressed in the mouse central nervous system. In adult brains, msvop was highly expressed in the hippocampus and cerebellum. When the gene was transfected into HEK293 cells in a GFP fusion vector, the protein was specifically localized in the cytosol. These results indicate that msvop is a central nervous system-specific gene and could be utilized for elucidating gene regulation in neuronal cells.
在本研究中，我们通过数字差异显示技术鉴定并表征了一个在小鼠脑内富集的单基因（msvop）。Msvop是非洲爪蟾突触囊泡2相关蛋白（svop）的小鼠同源物，其分子特征尚不清楚。3125 bp的全长cDNA编码一个约60 kDa的548个氨基酸的蛋白质。在NG108 - 15和HEK293细胞的 - 200至 - 100 bp区域发现了一个强启动子元件。逆转录聚合酶链反应（RT-PCR）和原位杂交分析证实msvop在小鼠中枢神经系统中严格表达。在成年大脑中，msvop在海马体和小脑中高度表达。当该基因以绿色荧光蛋白（GFP）融合载体转染到HEK293细胞中时，该蛋白特异性定位于细胞质中。这些结果表明msvop是一个中枢神经系统特异性基因，可用于阐明神经元细胞中的基因调控。
