Murray B E, Singh K V, Markowitz S M, Lopardo H A, Patterson J E, Zervos M J, Rubeglio E, Eliopoulos G M, Rice L B, Goldstein F W
Center for Infectious Diseases, University of Texas Medical School, Houston 77030.
J Infect Dis. 1991 Apr;163(4):780-5. doi: 10.1093/infdis/163.4.780.
Beta-lactamase-producing (Bla+) enterococci have been reported in three state and two countries. Pulsed-field gel electrophoresis was used to compare 14 Bla+ Enterococcus (Streptococcus) faecalis isolated from hospitalized patients in seven states and three continents. The restriction endonuclease digestion patterns of isolates from Connecticut, Massachusetts, Lebanon, and Argentina were all markedly different, indicating that these were different strains. However, isolates from Delaware, Texas, Pennsylvania (Philadelphia and Pittsburgh), Florida, and Virginia were similar, indicating that these isolates were derivatives of a single strain. This conclusion was supported by hybridization using individual fragments as probes. Spread of Bla+ enterococci within the hospital setting was also demonstrated. These findings illustrate the value of pulsed-field gel electrophoresis for epidemiologic analyses and support the importance of identifying and containing organisms with new resistance properties in an effort to decrease their transmission to and from, as well as within, hospitals.
已在三个州和两个国家报告了产β-内酰胺酶（Bla+）的肠球菌。采用脉冲场凝胶电泳对从七个州和三大洲的住院患者中分离出的14株产β-内酰胺酶粪肠球菌（粪链球菌）进行比较。来自康涅狄格州、马萨诸塞州、黎巴嫩和阿根廷的分离株的限制性内切酶消化模式均明显不同，表明这些是不同的菌株。然而，来自特拉华州、得克萨斯州、宾夕法尼亚州（费城和匹兹堡）、佛罗里达州和弗吉尼亚州的分离株相似，表明这些分离株是单一菌株的衍生物。使用单个片段作为探针进行杂交也支持了这一结论。还证明了Bla+肠球菌在医院环境中的传播。这些发现说明了脉冲场凝胶电泳在流行病学分析中的价值，并支持识别和控制具有新耐药特性的微生物的重要性，以努力减少它们在医院内外的传播。
