Harzallah Olfa, Kerkeni Abdelhamid, Baati Tarek, Mahjoub Silvia
Internal Medicine Department, Fattouma Bourguiba Hospital Monastir, Tunisia.
Eur J Intern Med. 2008 Nov;19(7):541-7. doi: 10.1016/j.ejim.2008.08.001. Epub 2008 Sep 11.
To study the oxidant/antioxidant status in Behçet's disease (BD) patients and the potential link between antioxidant enzymatic defences impairment and the disease duration, activity and severity.
40 BD patients (27 males, 13 females; mean age: 38.8 years) were prospectively enrolled in the study and compared to a sex and age matched control group. Plasma malondialdehyde (MDA), the reduced glutathione (GSH)/oxidised glutathione (GSSG) ratio, erythrocyte superoxide dismutase, catalase and glutathione peroxidase (GSH-PX) were analysed in both groups. A correlation analysis was performed between these parameters and disease duration, activity and severity. Activity and severity of BD were assessed meaning two respective clinical scores.
When compared to controls MDA was increased and GSH/GSSG reduced in BD patients (respectively p<0.005). Superoxide dismutase (SOD) was significantly lower while catalase was significantly higher in BD than in controls. Correlation analysis showed that SOD activity was significantly and negatively correlated to disease duration and activity but not to severity. Other oxidant/antioxidant markers were not significantly linked to neither disease duration nor activity and severity.
Our study confirm the existence of an oxidative stress (OS) state in BD as shown by the increase of MDA and the diminution of GSH/GSSG ratio which can be used as another index of OS. Despite this OS state, the activity of antioxidant enzymes and especially of SOD is impaired and negatively correlated to the disease duration and activity. We think that a rational strengthening of antioxidant defences should be part of an optimal treatment strategy.
研究白塞病（BD）患者的氧化/抗氧化状态，以及抗氧化酶防御功能受损与疾病持续时间、活动度和严重程度之间的潜在联系。
前瞻性纳入40例BD患者（27例男性，13例女性；平均年龄：38.8岁），并与性别和年龄匹配的对照组进行比较。分析两组患者血浆丙二醛（MDA）、还原型谷胱甘肽（GSH）/氧化型谷胱甘肽（GSSG）比值、红细胞超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶（GSH-PX）。对这些参数与疾病持续时间、活动度和严重程度进行相关性分析。通过两个各自的临床评分评估BD的活动度和严重程度。
与对照组相比，BD患者的MDA升高，GSH/GSSG降低（分别为p<0.005）。BD患者的超氧化物歧化酶（SOD）显著低于对照组，而过氧化氢酶显著高于对照组。相关性分析表明，SOD活性与疾病持续时间和活动度显著负相关，但与严重程度无关。其他氧化/抗氧化标志物与疾病持续时间、活动度和严重程度均无显著关联。
我们的研究证实BD患者存在氧化应激（OS）状态，表现为MDA升高和GSH/GSSG比值降低，这可作为OS的另一个指标。尽管存在这种OS状态，但抗氧化酶尤其是SOD的活性受损，并与疾病持续时间和活动度负相关。我们认为合理增强抗氧化防御应成为最佳治疗策略的一部分。
