Wang Chun-Ren, Li Li, Ni Hong-Bo, Zhai Yan-Qing, Chen Ai-Hua, Chen Jia, Zhu Xing-Quan
College of Animal Science and Technology, Heilongjiang August-First Land Reclamation University, Heilongjiang Province, People's Republic of China.
Exp Parasitol. 2009 Feb;121(2):193-7. doi: 10.1016/j.exppara.2008.10.012. Epub 2008 Nov 5.
In the present study, samples representing Orientobilharzia turkestanicum from cattle, sheep, cashmere goat and goat in Heilongjiang Province, China, were characterized and grouped genetically by sequences of internal transcribed spacer (ITS, including ITS-1 and ITS2) and 28S ribosomal DNA (28S rDNA). The ITS and 28S rDNA were amplified by polymerase chain reaction (PCR) and then sequenced and compared with that of other members of the Schistosomatidae available in GenBank, and phylogenetic relationships between them were re-constructed using the neighbor-joining and maximum parsimony methods. The lengths of ITS-1, ITS-2 and 28S rDNA sequences for all O. turkestanicum samples from different hosts were 384bp, 331bp and 1304bp, respectively. While the ITS-1 sequences of O. turkestanicum from each of the four different hosts, and ITS-2 of O. turkestanicum from cattle, sheep and cashmere goat were identical, respectively, the ITS-2 of O. turkestanicum from goat differed from that of O. turkestanicum from cattle, sheep and cashmere goat by one nucleotide. The 28S rDNA sequences of O. turkestanicum from sheep and cashmere goat were identical, but differed from that of O. turkestanicum from cattle and goat by two nucleotides, with the latter two also having identical 28S rDNA sequence. Phylogenetic analyses based on the combined sequences of the ITS-1 and ITS-2, or the 28S rDNA sequences placed O. turkestanicum within the genus Schistosoma, and it was phylogenetically closer to the African schistosome group than to the Asian schistosome group. These results should have implications for studying the origin and evolution of O. turkestanicum and other members of the Schistosomatidae.
在本研究中，对采自中国黑龙江省牛、绵羊、绒山羊和普通山羊的土耳其斯坦东毕吸虫样本，通过内转录间隔区（ITS，包括ITS-1和ITS2）和28S核糖体DNA（28S rDNA）序列进行了遗传特征分析和分组。通过聚合酶链反应（PCR）扩增ITS和28S rDNA，然后进行测序，并与GenBank中现有的裂体科其他成员进行比较，使用邻接法和最大简约法重建它们之间的系统发育关系。来自不同宿主的所有土耳其斯坦东毕吸虫样本的ITS-1、ITS-2和28S rDNA序列长度分别为384bp、331bp和1304bp。虽然来自四种不同宿主的土耳其斯坦东毕吸虫的ITS-1序列，以及来自牛、绵羊和绒山羊的土耳其斯坦东毕吸虫的ITS-2序列分别相同，但来自山羊的土耳其斯坦东毕吸虫的ITS-2与来自牛、绵羊和绒山羊的土耳其斯坦东毕吸虫的ITS-2在一个核苷酸上存在差异。来自绵羊和绒山羊的土耳其斯坦东毕吸虫的28S rDNA序列相同，但与来自牛和山羊的土耳其斯坦东毕吸虫的28S rDNA序列在两个核苷酸上存在差异，后两者的28S rDNA序列也相同。基于ITS-1和ITS-2的组合序列或28S rDNA序列的系统发育分析将土耳其斯坦东毕吸虫置于血吸虫属内，并且在系统发育上它与非洲血吸虫组比与亚洲血吸虫组更接近。这些结果对于研究土耳其斯坦东毕吸虫和裂体科其他成员的起源和进化具有重要意义。
